PureCube 100 NTA Agarose
The His tag is the most widely used affinity tag due to its small size, low immunogenicity, and versatility under native or denaturing conditions, as well as in presence of detergents and many other additives. Cube Biotech introduces the novel PureCube 100 NTA Agarose, based on high quality crosslinked agarose with 100 µm mean diameter, providing high flow rates in batch and FPLC applications. Due to the excellent flow properties of PureCube 100 Agarose, combined with the high capacity NTA ligand, high dynamic yield and protein purity can be achieved. PureCube 100 NTA Agarose can be loaded with transition metals to obtain different specificities for his-tagged proteins, or for other applications such as purification of phosphorylated proteins. PureCube 100 NTA Agarose is provided as a 50% suspension.
Why PureCube 100 NTA Agarose?
100 µm agarose beads for high flow rates in batch and FPLC
Can be loaded with most transition metals
Can be regenerated for re-use
-> Unsure which His affinity product to choose? See our overview.