Immobilized metal affinity chromatography, or IMAC, has been employed for many applications making use of its versatile interaction with aromatic nitrogens and phosphates. To this end, NTA matrices have been loaded with a broad range of transition metals, as optimized for the individual interaction. Al-NTA Agarose is often used for the purification of phosphorylated proteins. Alternative matrices are Fe-, Ga- or Zr-NTA.
PureCube Al-NTA Agarose provides:
- Ready-to-use matrix
- Purification of phosphorylated proteins
- Al-NTA MagBeads also available
Purification of phosphorylated proteins
IMAC methods, in particular Al-IDA, Fe-NTA or Ga-NTA, have been widely used to enrich phosphoproteins and phosphopeptides as part of the sample preparation for mass spectrometry (1,3,4). Depending on the sample, and the kind of phosphorylated proteins to be analyzed, also other transition metals, such as zirconium or aluminium have been loaded on NTA or IDA matrices for enrichment (5). Also, magnetic beads have been useful for this kind of sample preparation (2).
||Specific binding and purification of phosphorylated proteins
||Affinity phosphorylated proteins
|Metal Ion capacity
||15 µeqv Al3+/mL.
||pH 2-14, 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
||Delivered as a 50 % suspension