Al-NTA Agarose


Special Purification Resins
Immobilized metal affinity chromatography, or IMAC, has been employed for many applications making use of its versatile interaction with aromatic nitrogens and phosphates. To this end, NTA matrices have been loaded with a broad range of transition metals, as optimized for the individual interaction. Al-NTA Agarose is often used for the purification of phosphorylated proteins. Alternative matrices are Fe-, Ga- or Zr-NTA.

PureCube Al-NTA Agarose provides:

  • Ready-to-use matrix
  • Purification of phosphorylated proteins
  • Al-NTA MagBeads also available

Purification of phosphorylated proteins

IMAC methods, in particular Al-IDA, Fe-NTA or Ga-NTA, have been widely used to enrich phosphoproteins and phosphopeptides as part of the sample preparation for mass spectrometry (1,3,4). Depending on the sample, and the kind of phosphorylated proteins to be analyzed, also other transition metals, such as zirconium or aluminium have been loaded on NTA or IDA matrices for enrichment (5). Also, magnetic beads have been useful for this kind of sample preparation (2).

Features

Usage Specific binding and purification of phosphorylated proteins
Specifity Affinity phosphorylated proteins
Metal Ion capacity 15 µeqv Al3+/mL.
Stability pH 2-14, 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
Filling quantity Delivered as a 50 % suspension
Bead size 40 μm
Bead Ligand Al-NTA
Special Purification Resins
Immobilized metal affinity chromatography, or IMAC, has been employed for many applications making use of its versatile interaction with aromatic nitrogens and phosphates. To this end, NTA matrices have been loaded with a broad range of transition metals, as optimized for the individual interaction. Al-NTA Agarose is often used for the purification of phosphorylated proteins. Alternative matrices are Fe-, Ga- or Zr-NTA.

PureCube Al-NTA Agarose provides:

  • Ready-to-use matrix
  • Purification of phosphorylated proteins
  • Al-NTA MagBeads also available

Purification of phosphorylated proteins

IMAC methods, in particular Al-IDA, Fe-NTA or Ga-NTA, have been widely used to enrich phosphoproteins and phosphopeptides as part of the sample preparation for mass spectrometry (1,3,4). Depending on the sample, and the kind of phosphorylated proteins to be analyzed, also other transition metals, such as zirconium or aluminium have been loaded on NTA or IDA matrices for enrichment (5). Also, magnetic beads have been useful for this kind of sample preparation (2).

Features

Usage Specific binding and purification of phosphorylated proteins
Specifity Affinity phosphorylated proteins
Metal Ion capacity 15 µeqv Al3+/mL.
Stability pH 2-14, 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
Filling quantity Delivered as a 50 % suspension
Bead size 40 μm
Bead Ligand Al-NTA
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PureCube Al-NTA Agarose PureCube Al-NTA Agarose
Article number: 31403-Al
Al-NTA Agarose
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PureCube Al-NTA Agarose PureCube Al-NTA Agarose
NTA Agarose resin, loaded with aluminium (III) chloride for the purification of phosphorylated proteins. ★50% concentration★
Article number: 31403-Al
Sales price: From €104.00 *
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