Affinity chromatography is typically done with agarose beads with particle sizes of 40-150 µm. This material is very well suited for gravity flow and FPLC applications. Cube Biotech offers PureCube and PureCube 100 agaroses with a large variety of modifications that cover most applications. However, for specialized experiments, agaroses with exceptionally large bead sizes (300-500 µm in diameter) can be useful. For example, large beads settle fast by gravity and are therefore useful for automated purification procedures. Please note that due to the significantly different surface:volume ratio, binding capacity is usually lower than for standard sized beads.
PureCube Agarose XL beads provide:
XL sized Agarose compared to normal sized ones
Fig.2: Size distribution of PureCube, PureCube 100 and XL agaroses. Circles represent bead sizes drawn to scale.