Activated Agaroses XL


Agarose Guide
Affinity chromatography is typically done with agarose beads with particle sizes of 40-150 µm. This material is very well suited for gravity flow and FPLC applications. Cube Biotech offers PureCube and PureCube 100 agaroses with a large variety of modifications that cover most applications. However, for specialized experiments, agaroses with exceptionally large bead sizes (300-500 µm in diameter) can be useful. For example, large beads settle fast by gravity and are therefore useful for automated purification procedures. Please note that due to the significantly different surface:volume ratio, binding capacity is usually lower than for standard sized beads. Activated Agaroses can be used to couple molecules of your choice in your laboratory. Our careful in-house production provides low lot-to-lot variation, making activated agaroses also suitable for commercial production.

PureCube Activated Agaroses XL provide:

  • Pre-activated matrices for coupling to proteins, peptides, or antibodies
  • Easy to follow protocols and expert technical support
  • Highly reproducible synthesis in our own laboratories
  • High throughput protein purification: Large beads settle fast in 96 well plates
  • Compatible with the use of crude lysates
 
 
Table 1: Activated XL Agarose Resins in detail
Coupling mechanismCoupling ChemistryPureCube Activated Agarose XL
Covalent, via amine groups EDC/NHS Carboxy Agarose XL
Covalent, via amine groups Easy coupling of antibodies direct Fast and easy protocol NHS-activated Agarose XL
Covalent, via cysteine groups direct Maleimide-activated Agarose XL
Coupling of thermostable proteins and small molecules via amine and thiol direct Epoxy-activated Agarose XL
Covalent, via carboxy groups direct Amine-activated Agarose XL
Agarose Guide
Affinity chromatography is typically done with agarose beads with particle sizes of 40-150 µm. This material is very well suited for gravity flow and FPLC applications. Cube Biotech offers PureCube and PureCube 100 agaroses with a large variety of modifications that cover most applications. However, for specialized experiments, agaroses with exceptionally large bead sizes (300-500 µm in diameter) can be useful. For example, large beads settle fast by gravity and are therefore useful for automated purification procedures. Please note that due to the significantly different surface:volume ratio, binding capacity is usually lower than for standard sized beads. Activated Agaroses can be used to couple molecules of your choice in your laboratory. Our careful in-house production provides low lot-to-lot variation, making activated agaroses also suitable for commercial production.

PureCube Activated Agaroses XL provide:

  • Pre-activated matrices for coupling to proteins, peptides, or antibodies
  • Easy to follow protocols and expert technical support
  • Highly reproducible synthesis in our own laboratories
  • High throughput protein purification: Large beads settle fast in 96 well plates
  • Compatible with the use of crude lysates
 
 
Table 1: Activated XL Agarose Resins in detail
Coupling mechanismCoupling ChemistryPureCube Activated Agarose XL
Covalent, via amine groups EDC/NHS Carboxy Agarose XL
Covalent, via amine groups Easy coupling of antibodies direct Fast and easy protocol NHS-activated Agarose XL
Covalent, via cysteine groups direct Maleimide-activated Agarose XL
Coupling of thermostable proteins and small molecules via amine and thiol direct Epoxy-activated Agarose XL
Covalent, via carboxy groups direct Amine-activated Agarose XL
Activated Agaroses XL
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PureCube NHS Activated Agarose XL PureCube NHS Activated Agarose XL
20 ml 50% NHS-Activated Agarose, for direct coupling of biomolecules via amine groups. Medium particle size of 400 micrometer, for special applications
Article number: 58303
Sales price: From €140.00 *
PureCube Epoxy Activated Agarose XL PureCube Epoxy Activated Agarose XL
Lyophilized resin for 20 ml 50% Epoxy activated Agarose►For coupling of biomolecules via amine/thiol groups. Diameter of ~400µm.
Article number: 58703
Sales price: From €129.00 *
PureCube Carboxy Agarose XL PureCube Carboxy Agarose XL
1 ml 25% Carboxy Magnetic Beads, for coupling of biomolecules via amine groups using EDC/NHS
Article number: 58103
Sales price: From €108.00 *
PureCube Amine Activated Agarose XL PureCube Amine Activated Agarose XL
20 ml 50% Amine-Activated Agarose, for coupling of biomolecules via carboxy groups using EDC/NHS. Medium particle size of 400 micrometer, for special applications
Article number: 59005
Sales price: From €515.00 *
PureCube Maleimide Activated Agarose XL PureCube Maleimide Activated Agarose XL
20 ml 50% Maleimide-Activated Agarose, for direct, irreversible coupling of biomolecules via thiol groups. Medium particle size of 400 micrometer, for special applications
Article number: 59103
Sales price: From €387.00 *
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