Affinity tag antibodies

Recombinant proteins carrying an affinity tag can be easily detected using tag-specific antibodies, thereby eliminating the need for protein-specific antibodies for each protein.

 

Cube Biotech offers highly sensitive antibodies recognizing the three most commonly used affinity tags. All our antibodies are mouse monoclonal, and can be detected using the same secondary antibody, thereby facilitating processes.

 

Sets of affinity resins and matching antibodies are available as cost-effective combinations.

Table 1 shows technical and performance parameters of our three tag-specific antibodies.

Cube Affinity tag antibodies
 

 

PureCube Antibodies provide:

Highly specific and sensitive detection

Recognizing His, Rho1D4, Strep, and GST tags

Suitable for Western Blot and ELISA

A perfect match to PureCube Agaroses and MagBeads

 

 

Learn more about Affinity Agaroses
   
 
    PentaHis   Rho1D4   GST    Strep
Epitope   HHHHH   TETSQVAPA   Glutathione S-transferase from Schistosoma japonicum   Strep-tag II

Antibody type

  mouse monoclonal   mouse monoclonal   mouse monoclonal   mouse monoclonal
Detection limit in Western Blot (chemiluminescent detection)   50 pg   n.d.   500 pg   n.d.
Detection limit in Western Blot (chromogenic detection)   2 ng   n.d.   n.d.   5 ng

Recommended

dilution

  1:1000   1:1000   1:1000   200 ng/mL for chromogenic detection

Recommended

secondary

antibody

  e.g. goat anti mouse   e.g. goat anti mouse   e.g. goat anti mouse   e.g. rabbit anti mouse
Packaging  

100 µg

lyophilized
 

 

200 µg

in solution
 

100 µg

lyophilized
 

100 µg

lyophilized
Comment   BSA-free   -   -   HRP-conjugate also available
 
Table 1. Overview on technical features and performance of Cube Affinity tag antibodies.

 

 

 

 

 

Penta His detection of GFP protein

Fig.1: Western Blot ECL detection of purified his-tagged GFP using the Cube PentaHis antibody.
SDS-PAGE was done with defined amounts. Lanes 1: 50 pg, 2: 500 pg, 3: 1 ng, 4: 100 ng, 5: 250 ng, 6: 500 ng. Dilution factors: Primary antibody: 1:2000; secondary antibody 1:5000.
 

High sensitivity and specificity of PentaHis Antibody

 

The PentaHis antibody detects his-tagged proteins with high sensitivity and specificity. Background levels are low, and unambiguous signals are obtained, even at low protein concentrations.

 

Fig. 1 shows western blot detection of his-tagged green fluorescent protein (GFP) that was applied to an SDS-PAGE in defined amounts. 100 ng of protein are clearly visualized by chemiluminescent detection.

 

Note that detection levels are dependent on individual proteins, blot efficiency, antibody dilution, and choice of secondary antibody. Therefore, the optimal values (Table 1) are not always reached.

 
         

 

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Affinity tag antibodies

Cube Biotech Rho1D4 Antibody
Current Stock Level

Rho1D4 antibody (0.2 mg)

Sales price: 265,00 €

Product details

Cube Biotech PentaHis Antibody
Current Stock Level

PentaHis antibody, BSA-free (0.1 mg)

Sales price: 417,00 €

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Cube Biotech GST Antibody
Current Stock Level

GST antibody (0.1 mg)

Sales price: 452,00 €

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No image set
Current Stock Level

Strep antibody (0.1 mg)

Sales price: 183,00 €

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Current Stock Level

Strep antibody HRP

Sales price: 231,00 €

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