PureCube 1-step batch Columns

Empty 1-step batch columns are perfectly suited for batch purifications. They can be filled with your affinity resin of choice, and allow for binding, washing, and elution in one single spin column.

Designed for small to mid-scale protein purification, the PureCube 1-step batch spin columns save time and pipetting steps.

 

Featuring SelfSeal membrane technology, the column retains resin and sample in a chamber for batch incubation. By centrifugation, the membrane pores dilate and the filtered eluate gathers in the collection chamber of the column.

 

PureCube 1-step batch spin columns are available in two sizes: Mini for expression trials, small-scale screening, and other small-volume purification needs; or Midi Plus for volumes of up to 20 mL.

PureCube 1-step batch Columns
 

 

1-step batch Columns are useful for:

Easy and fast protein purification

Require only standard centrifuges

Multiple purifications in parallel

Fully compatible with PureCube Affinity Purification Matrices

 

 

Learn more about PureCube Agaroses

   

 

PureCube 1-step batch mini column, Cube Biotech   PureCube 1-step batch midi plus column, Cube Biotech

Fig.1: PureCube 1-step batch Mini (left) and Midi Plus columns.

 

 

Table 1: Technical features of PureCube 1-step batch columns.

 

    PureCube 1-step batch Mini   PureCube 1-step batch Midi Plus

  Body material

  Polypropylene   Polypropylene
  Filter pore size  

0.1-0.2 µm

low binding PVDF

 

0.1-0.2 µm

low binding PVDF

  Max. volume   600 µL   20 mL
  Max. g force  

12,000-14,000 x g

(45-degree fixed angle rotor)

 

1,500-2,000 x g

(swing-bucket rotor)

  Min. g force   2,500 x g for 1 min   100-200 x g for 1-10 min
  Typical spin times   0.5-1 min at 12-14,000 x g   5-10 min at 400 x g
 

 

 

 

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Batch Spin Columns

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PureCube 1-step batch Mini (50)

Sales price: 96,00 €

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PureCube 1-step Batch Mini column
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PureCube 1-step batch Mini (100)

Sales price: 172,00 €

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PureCube 1-step Batch MidiPlus column
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PureCube 1-step batch Midi Plus (8)

Sales price: 43,00 €

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PureCube 1-step batch Midi Plus (48)

Sales price: 222,00 €

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