PureCube Glutathione MagBeads

Magnetic beads are useful to purify proteins from dilute solutions, or when only low expression levels are achieved. PureCube Glutathione MagBeads are ferrimagnetic agarose beads coupled to glutathione, an efficient ligand for GST fusion proteins.

PureCube MagBeads are produced in a very robust, reproducible way to minimize lot-to-lot variability. (Fig.1) Our MagBeads are homogeneous in size, and provide the identical surface chemistry as PureCube Glutathione Agarose, making magnetic beads an excellent tool for fast screening of binding conditions. PureCube Glutathione MagBeads are provided as a 25% suspension.

PureCube Affinity MagBeads

 

PureCube Glutathione MagBeads provide:

  •         Binding capacity of up to 12 mg protein per ml settled beads
  •          purification of proteins expressed at low levels or from diluted solutions
  •         optimal conditions for pull-down experiments
  •         low unspecific binding
  •         Also available as Glutathione Agarose and prepacked cartridges

 

 

  • Comparison of 3 independent lots of PureCube Glutathione Magnetic Beads

     

    Fig.1.: Reliable lot-to-lot reproducibility. Glutathione-S-transferase
    was purified from E.coli lysates using three independent production
    lots of PureCube Glutathione MagBeads.
    FT: Flow through, E1-E5: Elution fractions.
  •  

PureCube GST Affinity MagBeads

MagBeads Knowledge Page

 

 
    Cube Biotech   Supplier P   Supplier S
Material   Magnetite (Fe3O4) beads coated with 6 % cross-linked agarose   Iron oxide particles encapsulated in crosslinked agarose   4% beaded agarose

Affinity ligand

  Glutathione covalently coupled to Agarose via a neutral 12 atom spacer   Reduced glutathione (GSH) covalently attached via sulfide linkage using a 12-atom linker   Glutathione covalently attached through the sulfur to epoxy-activated agarose resulting in a 12 atom spacer
Magnetization   ferrimagnetic   superparamagnetic   paramagnetic
Bead diameter   25-30 µm   1 to 10 µm   20-75 µm
Binding capacity   12 mg GST/mL of settled beads   5 to 10 mg GST/mL of settled beads   ≥15 μmol/mL agarose
Concentration   25% slurry in 20% ethanol   25% slurry in 20% ethanol   50% slurry in 30% ethanol
 
Table 1: Overview of commercially available Glutathione MagBeads.

 

  From Oct 1-Dec 31, 2017, buy this product with a 20% discount.

Quote "Cube2017" when placing your order. Learn more

 

Select Country
Results 1 - 3 of 3

GST Affinity MagBeads

PureCube Glutathione MagBeads
Current Stock Level

PureCube Glutathione MagBeads (1 ml)

Sales price: 48,00 €

Product details

PureCube Glutathione MagBeads
Current Stock Level

PureCube Glutathione MagBeads (5ml)

Sales price: 193,00 €

Product details

PureCube Glutathione MagBeads
Current Stock Level

PureCube Glutathione MagBeads (25 ml)

Sales price: 723,00 €

Product details