PureCube Co-NTA MagBeads

The His tag is the most widely used affinity tag due to its small size and versatility under native and denaturing conditions, as well as in presence of detergents and many other additives.

 

Magnetic beads are ideal for protein purification from dilute supernatants and for pull-down experiments. The agarose surface of our MagBeads is identical to that of PureCube Agarose, making them an ideal combination for small-scale screening and upscale reactions.

 

In addition to the widely used Ni-NTA MagBeads, Cube Biotech offers high-performance PureCube Co-NTA MagBeads for purification of his-tagged proteins. Specificity of this transition metal for histidine stretches is typically higher than that of nickel (see Fig. 1).

 

PureCube Co-NTA MagBeads are ferrimagnetic agarose beads coupled to an NTA chelating ligand, loaded with cobalt ions. PureCube Co-NTA MagBeads are delivered as a 25% suspension.

From Oct 1 to Dec 31, 2018, mention promo code "Cube2018" when placing your order, and save!

PureCube Agarose Magnetic Beads

PureCube His Affinity Magnetic Beads

 

 

Why PureCube Co-NTA MagBeads?

Learn more about Alternative IMAC Magnetic Beads

Learn more about Magnetic Beads

     

Fig. 3: Affinity and specificity of metal ions commonly used for IMAC. Loading an IDA or NTA resin with different metal ions can adjust the affinity and specificity of the resin to optimize the purity and yield of a purified protein.

Fig. 1: Affinity and specificity of metal ions commonly used for IMAC. Loading an IMAC resin with different metal ions can adjust the affinity and specificity to optimize the purity and yield of a purified protein.
 

Different metal ions confer different binding affinity and specificity

Loading different metal ions to a resin results in differing affinity and specificity for a his-tagged protein (Fig.1). Generally, cobalt exhibits the higest binding specificity of commonly used IMAC metal ions, leading to relatively low yields but high purity. Copper, at the other end of the spectrum, has a high affinity leading to high yields but unspecific binding. In searching for the optimal resin to purify a protein, it is recommended to explore different chelating ligands (IDA or NTA) and different metal ions.

     

Highest yield and specificity obtained with PureCube Ni-NTA and Co-NTA Magbeads

Fig.2: Highest yield and specificity obtained with PureCube Ni-NTA MagBeads. SDS-PAGE of radiolabeled, his-tagged proteins purified from an in vitro transcription/translation reaction with PureCube NTA MagBeads and comparable products from other suppliers.Rxn: total reaction, T Co: Talon Cobalt MagBeads 10 µl 5%, C Ni: PureCube Ni-NTA MagBeads, 5 µl 5%; C Co: PureCube Co-NTA MagBeads, 5 µl 5%; 5 Ni: Ni-NTA MagBeads from Supplier 5, 10 µl 5% suspension. Note that only half the amount of PureCube Ni and Co-NTA MagBeads were employed in each reaction compared to competitor beads. Data kindly provided by Dr. Daniel Kraut,  Department of Chemistry, Villanova University, Pennsylvania, USA
 

Nickel vs Cobalt IMAC

Depending on applications, nickel or cobalt IMAC may be the purification matrix of choice. For example, in customer data shown in Fig. 2, radio-labeled, his-tagged proteins generated in an in vitro transcription/translation reaction were purified using Ni and Co IMAC magnetic beads from different suppliers. PureCube Ni-NTA Magbeads showed highest binding capacity and highest purity of the his-tagged protein, which was then used as a substrate for proteasome studies.

Select Country
Results 1 - 3 of 3

Co-NTA MagBeads

PureCube Co-NTA MagBeads
Current Stock Level

PureCube Co-NTA MagBeads (1 ml)

Sales price: 66,00 €

Product details

PureCube Co-NTA MagBeads
Current Stock Level

PureCube Co-NTA MagBeads (5 ml)

Sales price: 286,00 €

Product details

PureCube Co-NTA MagBeads
Current Stock Level

PureCube Co-NTA MagBeads (25 ml)

Sales price: 1070,00 €

Product details