PureCube INDIGO Ni-MagBeads

The His tag is the most widely used affinity tag due to its small size and versatility under native and denaturing conditions, as well as in presence of detergents and many other additives. Magnetic beads are ideal for protein purification from dilute supernatants and for pull-down experiments.

 

Cube Biotech introduces the novel PureCube INDIGO Ni-MagBeads - ferrimagnetic agarose beads coupled to our novel, proprietary INDIGO ligand, loaded with nickel ions.

 

Using the INDIGO ligand, purification of his-tagged proteins is possible in the presence of up to 20 mM DTT and 20 mM EDTA (Fig.1) at high protein capacity. 

 

Please note that the novel INDIGO ligand cannot be stripped with EDTA, or cleaned at high pH using NaOH. PureCube INDIGO Ni-MagBeads are delivered as a 25% suspension.

From Oct 1 to Dec 31, 2018, mention promo code "Cube2018" when placing your order, and save!

 

PureCube Affinity MagBeads

PureCube His Affinity MagBeadsPureCube INDIGO Ni-MagBeads

 

 

Why PureCube INDIGO Ni-MagBeads?

 

Stable in 20 mM DTT and 20 mM EDTA, at pH 4-9
High binding capacity (up to 80 mg/mL settled beads)
Compatible with eukaryotic cell culture media
Ideal for secreted proteins -  see our new protocol & save!
Also available as PureCube INDIGO Ni-Agarose

-> Unsure which His affinity product to choose? See our overview.

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PureCube MagBeads Knowledge

 

PureCube INDIGO Ni-Magbeads yield pure protein in the presence of DTT and EDTAPureCube INDIGO Ni-Magbeads: get high yields of protein in the presence of DTT and EDTA

Fig.1: Using PureCube INDIGO Ni-MagBeads, his-tagged proteins can be purified at high yield and purity even in the presence of 10 mM DTT and 20 mM EDTA. 15 mL E.coli lysate containing recombinant green fluorescent protein GFP (left) or human c-Jun N-terminal protein kinase 1 JNK1 (right) were applied to 250 µL PureCube INDIGO Ni-MagBeads each. Standard buffers containing 10 mM DTT and 20 mM EDTA were used throughout the purification procedure. Yields of 89 mg GFP and 48 mg JNK1 per mL MagBeads were obtained. MW: Molecular weight marker. L: Cleared lysate, FT: flow through, W: wash; E1-E3: elution fractions 1-3.

 

 

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INDIGO Ni-MagBeads

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PureCube INDIGO Ni-MagBeads (1 ml)

Sales price: 67,00 €

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PureCube INDIGO Ni-MagBeads (5 ml)

Sales price: 291,00 €

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PureCube INDIGO Ni-MagBeads (25 ml)

Sales price: 1094,00 €

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