PureCube Ni-IDA MagBeads

The His tag is the most widely used affinity tag due to its small size and versatility under native and denaturing conditions, as well as in presence of detergents and many other additives.

 

Magnetic beads are ideal for protein purification from dilute supernatants and for pull-down experiments. The agarose surface of our MagBeads is identical to that of PureCube Agarose, making them an ideal combination for small-scale screening and upscale reactions.

 

PureCube Ni-IDA MagBeads are ferrimagnetic agarose beads coupled to an IDA chelating ligand, loaded with nickel ions. PureCube Ni-NTA MagBeads are delivered as a 25% suspension.

 

For increased specificity, we offer a range of different His affinity resins, e.g. carrying the NTA ligand (what's the difference?). Please see our overview page for more information.

 

PureCube Affinity MagBeads

PureCube His Affinity MagBeadsPureCube Ni-IDA MagBeads

 

 

Why PureCube Ni-IDA MagBeads?

  •           High binding capacity (up to 70 mg/mL settled beads)
  •          Low unspecific binding
  •         Stable in up to 10 mM DTT and up to 1 mM EDTA (Not stable enough?)
  •         Ideal for purification from dilute solutions and for pulldown experiments
  •         Also available as Ni-IDA Agarose and prepacked cartridges

Alternative IMAC Products

MagBeads Knowledge Page

 

 

 

    Cube Biotech   Supplier G   Supplier Q
Material  

Magnetite (Fe3O4) beads

coated with 6% cross-

linked agarose

 

Highly cross-linked

spherical agarose

including magnetite

 

Agarose beads with

magnetic particles

Affinity ligand

 

Ni-IDA,

NTA loaded with

Ni, Co, Al, Cu or Zn

NEW: INDIGO Ni-MagBeads

  Ni-IMAC   Ni-NTA
Magnetization   ferrimagnetic   ferrimagnetic   ferrimagnetic
Bead diameter   25-30 µm   63 or 37-100 µm   20-70 µm
Binding capacity   up to 70 mg (IDA) or 80 mg (NTA/INDIGO) His-tagged protein/mL of settled beads  

50 mg His-tagged

protein/mL of settled beads

 

2 mg His-tagged

protein/ml suspension

(40 mg/ml settled beads)

Concentration   25% slurry in 20% ethanol   10 or 5% slurry in 20% ethanol   5% slurry in 30% ethanol
Table 1: Features of commercially available His affinity magnetic beads.

 

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Ni-IDA MagBeads

PureCube Ni-IDA MagBeads
Current Stock Level

PureCube Ni-IDA MagBeads (1 ml)

Sales price: 42,00 €

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PureCube Ni-IDA MagBeads
Current Stock Level

PureCube Ni-IDA MagBeads (5 ml)

Sales price: 164,00 €

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PureCube Ni-IDA MagBeads
Current Stock Level

PureCube Ni-IDA MagBeads (25 ml)

Sales price: 616,00 €

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