PureCube Ni-NTA MagBeads

The His tag is the most widely used affinity tag due to its small size and versatility under native and denaturing conditions, as well as in presence of detergents and many other additives.

Magnetic beads are ideal for protein purification from dilute supernatants and for pull-down experiments. The agarose surface of our MagBeads is identical to that of PureCube Agarose, making them an ideal combination for small-scale screening and upscale reactions.

PureCube Ni-NTA MagBeads are ferrimagnetic agarose beads coupled to an NTA chelating ligand, loaded with nickel ions. They are delivered as a 25% suspension.

 

PureCube Affinity MagBeads

PureCube His Affinity MagBeads

 

Why PureCube Ni-NTA MagBeads?

 

-> Unsure which His affinity product to choose? See our overview.

 


 

 

Alternative IMAC MagBeads

MagBeads Knowledge

 

 

 

    Cube Biotech   Supplier G   Supplier Q
Material   Magnetite (Fe3O4) beads coated with 6% cross-linked agarose   Highly cross-linked spherical agarose including magnetite  

Agarose beads with

magnetic particles

Affinity ligand

 

Ni-IDA,

NTA loaded with

Ni, Co, Al, Cu or Zn

NEW: INDIGO Ni-MagBeads


 

  Ni-IMAC   Ni-NTA
Magnetization   ferrimagnetic   ferrimagnetic   ferrimagnetic
Bead diameter   25-30 µm   63 or 37-100 µm   20-70 µm
Binding capacity   up to 70 mg (IDA) or 80 mg (NTA/INDIGO) His-tagged protein/mL of settled beads  

50 mg His-tagged

protein/mL of settled beads

 

2 mg His-tagged

protein/ml suspension

(40 mg/ml settled beads)

Concentration   25% slurry in 20% ethanol   10 or 5% slurry in 20% ethanol   5% slurry in 30% ethanol
Table 1: Features of commercially available His affinity magnetic beads.

 

 

>46% higher binding capacity of PureCube Ni-NTA MagBeads compared to competitor products  
Fig.1: >46% higher yield obtained with PureCube Ni-NTA MagBeads.
SDS-PAGE of GFP expressed in E.coli and purified with comparable amounts of PureCube Ni-NTA MagBeads and equivalent, market-leading magnetic beads.

 

  From Oct 1-Dec 31, 2017, buy this product with a 20% discount.

Quote "Cube2017" when placing your order. Learn more

 

Select Country
Results 1 - 3 of 3

Ni-NTA MagBeads

Ni-NTA MagBeads
Current Stock Level

PureCube Ni-NTA Mag Beads (1 ml)

Sales price: 59,00 €

Product details

Ni-NTA MagBeads
Current Stock Level

PureCube Ni-NTA Mag Beads (5 ml)

Sales price: 257,00 €

Product details

Ni-NTA MagBeads
Current Stock Level

PureCube Ni-NTA Mag Beads (25 ml)

Sales price: 965,00 €

Product details