PureCube Cu-NTA MagBeads

Immobilized metal affinity chromatography, or IMAC, has been employed for many applications making use of its versatile interaction with aromatic nitrogens and phosphates. To this end, NTA matrices have been loaded with a broad range of transition metals, as optimized for the individual interaction. Magnetic beads are ideal for protein purification from dilute supernatants and for pull-down experiments.

 

Cu-NTA MagBeads can be used for the purification of his-tagged proteins. They provide high yields of protein, at lower specificity than e.g. Ni- or Co-IMAC. Cu-NTA matrices have also been used for the enrichment of copper-binding proteins, in particular with plant extracts (1).

PureCube Cu-NTA MagBeads are delivered as a 25% suspension.

 

PureCube Agarose Magnetic Beads

PureCube Alternative IMAC Magnetic Beads

 

 

PureCube Cu-NTA MagBeads provide:

  •         Ready-to-use magnetic beads
  •          Enrichment of copper-binding proteins
  •         Purification of his-tagged proteins with different specificity
  •         Cu-NTA Agaroses also available

Learn more about Alternative IMAC Magnetic Beads

Learn more about Magnetic Beads

 

Fig. 3: Affinity and specificity of metal ions commonly used for IMAC. Loading an IDA or NTA resin with different metal ions can adjust the affinity and specificity of the resin to optimize the purity and yield of a purified protein.

Fig. 1: Affinity and specificity of metal ions commonly used for IMAC.
Loading an IMAC resin with different metal ions can adjust the affinity and specificity to optimize the purity and yield of a purified protein.
 

Different metal ions confer different binding affinity and specificity

Loading different metal ions to a resin results in differing affinity and specificity for a his-tagged protein. Generally, cobalt exhibits the higest binding specificity of commonly used IMAC metal ions, leading to relatively low yields but high purity. Copper, at the other end of the spectrum, has a high affinity leading to high yields but unspecific binding. In searching for the optimal resin to purify a protein, it is recommended to explore different chelating ligands (IDA or NTA) and different metal ions.

Literature references

1.
Kung. C.C. et al. Proteomic survey of copper-binding proteins in Arabidopsis roots by immobilized metal affinity chromatography and mass spectrometry. Proteomics (2006), 6(9)2746-58.

 

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Cu-NTA MagBeads

PureCube MagBeads
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PureCube Cu-NTA MagBeads (1 ml)

Sales price: 64,00 €

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PureCube MagBeads
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PureCube Cu-NTA MagBeads (5 ml)

Sales price: 278,00 €

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PureCube MagBeads
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PureCube Cu-NTA MagBeads (25 ml)

Sales price: 1039,00 €

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