Assembled nanodiscs - untagged

Assembled nanodiscs are available as part of a collaboration with the Institute of Biophysics at the University of Frankfurt. They are provided as convenient aliquots in 50 µL solutions of 0.5 mM concentration. End concentrations in the cell-free reaction range from 20-80 µM. Bacterial and eucaryotic membrane proteins can be expressed (Fig 1 and 2), activity may depend on the lipid composition of the assembled nanodisc. Alternatively, nanodiscs can be assembled from the pre-aliquoted components provided in our Nanodisc Assembly kits.

To accommodate his-tagged membrane proteins, assembled nanodiscs prepared from untagged membrane scaffold proteins (MSPs) are now available.

 

Please note that assembled nanodiscs are intended for use in cell-free expression reactions only. They are not recommended for the reconstitution of membrane proteins already solubilized in detergent, or for the solubilization of proteins from membranes.

 


Available assembled nanodisc variations:

Ideal in combination with E.coli cell-free expression lysates
DMPC and POPC as eukaryotic phospholipids
DMPG as prokaryotic phospholipid

MSP1D1 (wild-type), MSP1D1dH5, MSP2N2 and MSPE3D1 scaffold proteins, both his-tagged and untagged

Biotinylated nanodiscs and other lipid modifications available
 

 

 

Nanodisc products

 

  From Oct 1-Dec 31, 2017, buy these products with a 10% discount.

Quote "Cube2017" when placing your order. Learn more

 

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Assembled nanodiscs untagged

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Nanodisc MSP1D1_DMPC (50 uL)

Sales price: 511,00 €

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Nanodisc MSP1D1_POPC (50 uL)

Sales price: 511,00 €

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Nanodisc MSP1D1_DMPG (50 uL)

Sales price: 511,00 €

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Nanodisc MSP1D1 dH5_DMPC (50 uL)

Sales price: 511,00 €

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Nanodisc MSP1D1 dH5_POPC (50 uL)

Sales price: 511,00 €

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Nanodisc MSP1D1 dH5_DMPG (50 uL)

Sales price: 511,00 €

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Nanodisc MSP1E3D1_DMPC (50 uL)

Sales price: 511,00 €

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Nanodisc MSP1E3D1_POPC (50 uL)

Sales price: 511,00 €

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Nanodisc MSP1E3D1_DMPG (50 uL)

Sales price: 511,00 €

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Nanodisc MSP2N2_DMPC (50 uL)

Sales price: 628,00 €

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