PureCube Glutathione Cartridges and Compact Cartridges

The GST tag is a 26 kDa protein that binds to glutathione-coupled agarose resins. This tag is considerably larger than the His tag, Strep tag, or Rho1D4 tag, and is often chosen to promote recombinant protein folding.

 

Cube Biotech offers high-performance PureCube Glutathione Agarose, prepacked in affinity chromatography columns with 1 and 5 mL volume, ready to be used on automated liquid chromatography systems (e.g., FPLC).

PureCube Cartridges

 

 

PureCube Glutathione Cartridges provide:

  Ready-to-use cartridges

  Two cartridge geometries: Standard and Compact

  Protein binding capacity of up to 10 mg/mL

  Compatible with common chromatography instruments


 

 

 
 

PureCube Glutathione Cartridges

Learn more about PureCube Cartridges

 


GST-tagged protein purification with PureCube Glutathione Agarose


 

High protein binding capacity

In a comparative study with equivalent, market-leading resins, PureCube Glutathione Agarose proved to be a competitive alternative, exhibiting the same protein binding capacity. Figure 1 is an SDS-PAGE of GST purified on gravity columns packed with PureCube Glutathione Agarose and the equivalent resin from Competitor G. Both resins yielded up to 10 mg/mL protein. 

Fig. 1: Purification yield is comparable to that of a market-leading resin.SDS-PAGE of GST expressed in E.coli and purified in gravity columns with PureCube Glutathione Agarose and the equivalent resin from Competitor G. Up to 10 mg/ml protein yield was obtained with PureCube Glutathione Agarose (elution fractions E1-E6, Cube). TL: total lysate; CL: cleared lysate; FT: flow-through; W1/W4: wash fractions; E1-E6: elution fractions.
 

 

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GST Affinity Cartridges

PureCube Cartridge 1 mL
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PureCube Glutathione Cartridge (1x1 ml)

Sales price: 39,00 €

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PureCube Cartridge 1 mL
Current Stock Level

PureCube Glutathione Cartridge (5x1 ml)

Sales price: 182,00 €

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gst_-1-ml_450px_96dpi4
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PureCube Glutathione Cartridge 5 mL
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PureCube Glutathione Cartridge (1x5 ml)

Sales price: 149,00 €

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PureCube Glutathione Cartridge 5 mL
Current Stock Level

PureCube Glutathione Cartridge (5x5 ml)

Sales price: 701,00 €

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