PureCube His Affinity Cartridges and Compact Cartridges

The polyhistidine tag is the most widely used affinity tag due to its small size, low immunogenicity, and versatility under native and denaturing conditions, as well as in presence of detergents and many other additives. Taking advantage of the affinity of transition metal ions for the imidazole ring of histidine, immobilized metal affinity chromatography (IMAC) is used to purify his-tagged proteins.  Cube Biotech offers high-performance PureCube IDA Agarose and NTA Agarose, and the novel INDIGO Agarose, prepacked in affinity chromatography columns with 1 and 5 mL volume, ready to be used on automated liquid chromatography systems (e.g., FPLC).

PureCube Cartridges



PureCube His Affinity Cartridges and Compact Cartridges provide:

  Highest yields and specificity for his-tagged protein purification

  Two cartridge geometries: Standard and Compact

  Compatible with common chromatography instruments

  Cartridges with other transition metals available on request



PureCube 100 INDIGO Ni-CartridgesPureCube 100 Ni-NTA CartridgesPureCube 100 Co-NTA CartridgesPureCube Ni-NTA CartridgesPureCube Co-NTA CartridgesPureCube Ni-IDA Cartridges



PureCube His Affinity Cartridges

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 IDA vs. NTA. Chelating ligands nitrilotriacetic acid (NTA) and iminodiacetic acid (IDA) support similar interaction between Ni2+ and imidazole rings of a polyhistidine tag, but NTA coordinates the Ni2+ with 4 valencies and IDA with only 3 (orange circles). This difference impacts the quality of the resulting purified protein fraction.


Fig. 1. PureCube Ni-NTA Cartridge.
5 ml (left) and 1 ml sizes


Fig. 2. PureCube CompactCartridge Ni-NTA.
1 ml (top) and 5 ml sizes