PureCube Co-NTA Cartridges

The His tag is the most widely used affinity tag due to its small size, low immunogenicity, and versatility under native or denaturing conditions, as well as in presence of detergents and many other additives.

 

In addition to the widely used Ni-NTA Agarose, Cube Biotech offers high-performance PureCube Co-NTA Agarose, prepacked in affinity chromatography columns with 1 and 5 mL volume, ready to be used on automated liquid chromatography systems (e.g., FPLC). Specificity of this transition metal for histidine stretches is typically higher than that of nickel (see Fig. 1).

 

PureCube Cartridges

PureCube His Affinity CartridgesPureCube Co-NTA Cartridges

 

PureCube Co-NTA Cartridges provide:

  Optimal balance of yield and purity

  Superior DTT and EDTA stability

  compatibility with common chromatography instruments

  Can be regenerated for reuse

  Co-NTA Agarose and MagBeads also available

 

 

 

Learn more about IMAC

Learn more about PureCube Cartridges

 

 

     PureCube Cartridge, 1 mL   PureCube Cartridge, 5 mL
  Bed volume  

1 mL

 

5 mL

  Dimensions (mm)  

6.2 X 50

 

11 X 80

  Body material  

Polypropylene

 

Acrylate

  Inlet  

10-32 UNF female thread

 

10-31 UNF female thread

  Outlet   10-32 UNF female thread   10-32 UNF female thread

 

Table 1: Technical features of PureCube Cartridges.

 

                         

   

Fig. 3: Affinity and specificity of metal ions commonly used for IMAC. Loading an IDA or NTA resin with different metal ions can adjust the affinity and specificity of the resin to optimize the purity and yield of a purified protein.

Fig. 1: Affinity and specificity of metal ions commonly used for IMAC.
Loading an IMAC resin with different metal ions can adjust the affinity and specificity to optimize the purity and yield of a purified protein.
 

Different metal ions confer different binding affinity and specificity

Loading different metal ions to a resin results in differing affinity and specificity for a his-tagged protein. Generally, cobalt exhibits the higest binding specificity of commonly used IMAC metal ions, leading to relatively low yields but high purity. Copper, at the other end of the spectrum, has a high affinity leading to high yields but unspecific binding. In searching for the optimal resin to purify a protein, it is recommended to explore different chelating ligands (IDA or NTA) and different metal ions.

 

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Co-NTA Cartridges

PureCube Co-NTA Cartridge 1 mL
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PureCube Co-NTA Cartridge (1x1 ml)

Sales price: 35,00 €

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PureCube Co-NTA Cartridge 1 mL
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PureCube Co-NTA Cartridge (5x1 ml)

Sales price: 162,00 €

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PureCube Co-NTA Cartridge 5 mL
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PureCube Co-NTA Cartridge (1x5 ml)

Sales price: 109,00 €

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PureCube Co-NTA Cartridge 5 mL
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PureCube Co-NTA Cartridge (5x5 ml)

Sales price: 517,00 €

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