PureCube 100 Co-NTA Cartridges and Compact Cartridges

The His tag is the most widely used affinity tag due to its small size, low immunogenicity, and versatility under native or denaturing conditions, as well as in presence of detergents and many other additives.

Cube Biotech introduces the novel PureCube 100 Co-NTA Agarose, based on high quality crosslinked agarose with 100 µm mean diameter, providing high flow rates in batch and FPLC applications. Specificity of this transition metal for histidine stretches is typically higher than that of nickel (see Fig. 1).

 

Due to the excellent flow properties of PureCube 100 Agarose, combined with the high capacity NTA ligand, high dynamic yield and protein purity can be achieved.

Cube Biotech offers PureCube 100 Co-NTA Agarose, prepacked in affinity chromatography columns with 1 and 5 mL volume, ready to be used on automated liquid chromatography systems (e.g., FPLC).

 

PureCube Cartridges

PureCube His Affinity CartridgesPureCube 100 Co-NTA Cartridges

 

PureCube 100 Co-NTA Cartridges provide:

  High flow rates AND specificity for his-tagged proteins

  Can be regenerated for reuse

  Compatible with low pressure chromatography instruments

  Two cartridge geometries: Standard and Compact

  Stable in 10 mM DTT and 1 mM EDTA. Need to use higher concentrations?

 

-> Unsure which His affinity product to choose? See our overview.

 

                      

Learn more about PureCube Cartridges

 

Fig. 3: Affinity and specificity of metal ions commonly used for IMAC. Loading an IDA or NTA resin with different metal ions can adjust the affinity and specificity of the resin to optimize the purity and yield of a purified protein.

Fig. 1: Affinity and specificity of metal ions commonly used for IMAC.
Loading an IMAC resin with different metal ions can adjust the affinity and specificity to optimize the purity and yield of a purified protein.
 

Different metal ions confer different binding affinity and specificity

Loading different metal ions to a resin results in differing affinity and specificity for a his-tagged protein. Generally, cobalt exhibits the higest binding specificity of commonly used IMAC metal ions, leading to relatively low yields but high purity. Copper, at the other end of the spectrum, has a high affinity leading to high yields but unspecific binding. In searching for the optimal resin to purify a protein, it is recommended to explore different chelating ligands (IDA or NTA) and different metal ions.

 

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Co-NTA Cartridges 100

PureCube Co-NTA Cartridge 1 mL
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PureCube 100 Co-NTA Cartridge (1x1 ml)

Sales price: 49,00 €

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PureCube Co-NTA Cartridge 1 mL
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PureCube 100 Co-NTA Cartridge (5x1 ml)

Sales price: 197,00 €

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PureCube Co-NTA Cartridge 5 mL
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PureCube 100 Co-NTA Cartridge (1x5 ml)

Sales price: 164,00 €

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PureCube Co-NTA Cartridge 5 mL
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PureCube 100 Co-NTA Cartridge (5x5 ml)

Sales price: 672,00 €

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