PureCube 100 INDIGO Ni-Cartridges

The His tag is the most widely used affinity tag due to its small size, low immunogenicity, and versatility under native or denaturing conditions, as well as in presence of detergents and many other additives.

 

Cube Biotech introduces the novel PureCube 100 INDIGO Ni-Agarose, based on high quality crosslinked agarose with 100 µm mean diameter, providing high flow rates in batch and FPLC applications.

Using the novel, proprietary INDIGO ligand, purification of his-tagged proteins is possible in the presence of up to 20 mM DTT and 20 mM EDTA at a protein capacity considerably higher than for comparable competitor products.

 

The novel INDIGO ligand cannot be stripped with EDTA, or cleaned at high pH using NaOH. However, without any regeneration, PureCube 100 INDIGO Ni-Agarose was re-used eight times without reduction in performance (Fig. 1).

Cube Biotech offers PureCube 100 INDIGO Ni-Agarose, prepacked in affinity chromatography columns with 1 and 5 mL volume, ready to be used on automated liquid chromatography systems (e.g., FPLC).

 

PureCube Cartridges

PureCube His Affinity CartridgesPureCube 100 INDIGO Ni-Cartridges

 

Why PureCube 100 INDIGO Ni-Cartridges?

  High flow rates AND dynamic binding capacity

  Stable in 20 mM DTT and 20 mM EDTA, at pH 4-9

  Compatible with eukaryotic cell culture media

  Compatible with low pressure chromatography instruments

 

-> Unsure which His affinity product to choose? See our overview.

 

 

     PureCube Cartridge, 1 mL   PureCube Cartridge, 5 mL
  Bed volume  

1 mL

 

5 mL

  Dimensions (mm)  

6.2 X 50

 

11 X 80

  Body material  

Polypropylene

 

Acrylate

  Inlet  

10-32 UNF female thread

 

10-31 UNF female thread

  Outlet   10-32 UNF female thread   10-32 UNF female thread
Table 1: Technical features of PureCube Cartridges.

 

Learn more about PureCube Cartridges

 
PureCube 100 INDIGO Ni-Agarose can be re-used multiple times without regeneration. ChromatogramPureCube 100 INDIGO Ni-Agarose can be re-used multiple times without regeneration. SDS-PAGE showing purification fractions.
Fig.1: PureCube 100 INDIGO Ni-Agarose can be re-used multiple times without regeneration. GFP was spiked into E.coli lysates and purified in eight aliquots on the same 1 ml column filled with PureCube 100 INDIGO Ni-Agarose. Between each run, the column was briefly washed with loading buffer containing PBS and 10 mM imidazole. No decrease in performance was observed, even after eight consecutive runs. Left: Chromatogram; Right: SDS-PAGE. M: Marker, L: Lysate, S: Lysate spiked with GFP.
 

 

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INDIGO Ni-Cartridges 100

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PureCube 100 INDIGO Ni-Cartridge (5x1 ml)

Sales price: 183,00 €

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PureCube 100 INDIGO Ni-Cartridge (1x5 ml)

Sales price: 152,00 €

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PureCube 100 INDIGO Ni-Cartridge (5x5 ml)

Sales price: 621,00 €

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