PureCube 100 Ni-NTA Cartridges

The His tag is the most widely used affinity tag due to its small size, low immunogenicity, and versatility under native or denaturing conditions, as well as in presence of detergents and many other additives.

 

Cube Biotech introduces the novel PureCube 100 Ni-NTA Agarose, based on high quality crosslinked agarose with 100 µm mean diameter, providing high flow rates in batch and FPLC applications.

 

Due to the excellent flow properties of PureCube 100 Agarose, combined with the high capacity NTA ligand, high dynamic yield and protein purity can be achieved. Even at flow rates of 7 ml/min, more than 20 mg/ml his-tagged protein could be purified (Fig.1).

 

PureCube 100 Ni-NTA Agarose shows high affinity to his-tagged proteins. Recovery rates of 85-100% were achieved, even with low concentrated protein samples (Fig.2).

Cube Biotech offers PureCube 100 Ni-NTA Agarose, prepacked in affinity chromatography columns with 1 and 5 mL volume, ready to be used on automated liquid chromatography systems (e.g., FPLC).

 

PureCube Cartridges

PureCube His Affinity CartridgesPureCube 100 Ni-NTA Cartridges

 

PureCube 100 Ni-NTA Cartridges provide:

  High flow rates AND dynamic binding capacity

  Excellent recovery rates even from dilute solutions

  Can be regenerated for reuse

  Compatible with low pressure chromatography instruments

  Stable in 10 mM DTT and 1 mM EDTA. Need to use higher concentrations?

 

-> Unsure which His affinity product to choose? See our overview.

 

 

     PureCube Cartridge, 1 mL   PureCube Cartridge, 5 mL
  Bed volume  

1 mL

 

5 mL

  Dimensions (mm)  

6.2 X 50

 

11 X 80

  Body material  

Polypropylene

 

Acrylate

  Inlet  

10-32 UNF female thread

 

10-31 UNF female thread

  Outlet   10-32 UNF female thread   10-32 UNF female thread
Table 1: Technical features of PureCube Cartridges.

 

Learn more about PureCube Cartridges

   

 

High dynamic binding capacity of PureCube 100 Ni-NTA Agarose at up to 7 ml/min flow rates. ChromatogramHigh dynamic binding capacity of PureCube 100 Ni-NTA Agarose at up to 7 ml/min flow rates. SDS-PAGE

 
Fig. 1: High dynamic binding capacity of PureCube 100 Ni-NTA Agarose.  GFP was spiked into E.coli lysates and purified on a 1 mL PureCube Cartridge filled with PureCube 100 Ni-NTA Agarose at flow rates from 1 to 7 ml/min, and in a gravity flow batch procedure.
Left: Flow diagram, Right: SDS-PAGE analysis of elution fractions.

 

Excellent protein recovery with PureCube 100 Ni-NTA Agarose
 
 
 
 
Fig.2: Excellent protein recovery with PureCube 100 Ni-NTA Agarose.  GFP was spiked into E.coli lysates in different quantities and purified on a 1 mL PureCube Cartridge filled with PureCube 100 Ni-NTA Agarose. Recovered GFP was quantified by absorption at 488 nm.

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Ni-NTA Cartridges 100

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PureCube 100 Ni-NTA Cartridge (1x1 ml)

Sales price: 46,00 €

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PureCube 100 Ni-NTA Cartridge (5x1 ml)

Sales price: 183,00 €

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PureCube 100 Ni-NTA Cartridge (1x5 ml)

Sales price: 152,00 €

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PureCube 100 Ni-NTA Cartridge (5x5 ml)

Sales price: 621,00 €

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