Active, purified OPRM1 (Mu-type-opioid receptor) in nanodisc

Membrane proteins are the most pharmaceutically relevant protein class. At the same time, it is very difficult to obtain them in pure, active form. Cube Biotech's protein experts have worked hard to produce human GPCRs in sufficient quality that we can now offer to the community. We have reconstituted the proteins into nanodiscs for stabilization.

Our first available pure, active, nanodisc inserted membrane protein is OPRM1,  a human GPCR involved in opiate response, pain modulation and analgesia, but also breast cancer. The protein is ready for ligand binding studies, crystallization, and other biochemical/biophysical experiments.

 

Please contact us for bulk inquiries, academic projects or collaboration ideas.

 

Active Membrane Proteins

 

       
Fig. 1: SDS-PAGE of OPRM1 in nanodisc, purified via Rho1D4 affinity chromatography.
Orange arrow: GPCR; Green arrow: membrane scaffold protein
    Highly pure OPRM1-nanodisc preparation after Rho1D4 affinity chromatography
   

Protein

OPRM1 (Mu-type opioid receptor 1)

Alternative names

MOR1, MOP, Mu opiate receptor

UniProt

P35372

Protein class

GPCR, class A (Rhodopsin-like receptors)

Organism

Human (Homo sapiens)

Sequence

Full-length, wildtype sequence
Initial methionine (bold), spacer (gray), Rho1D4 tag (green)
 
M D S S A A P T N A S N C T D A L A Y S S C S P A P S P G S W V N L S H L D G N L S D P C G P N R T D L G G R D S L C P P T G S P S M I T A I T I M A L Y S I V C V V G L F G N F L V M Y V I V R Y T K M K T A T N I Y I F N L A L A D A L A T S T L P F Q S V N Y L M G T W P F G T I L C K I V I S I D Y Y N M F T S I F T L C T M S V D R Y I A V C H P V K A L D F R T P R N A K I I N V C N W I L S S A I G L P V M F M A T T K Y R Q G S I D C T L T F S H P T W Y W E N L L K I C V F I F A F I M P V L I I T V C Y G L M I L R L K S V R M L S G S K E K D R N L R R I T R M V L V V V A V F I V C W T P I H I Y V I I K A L V T I P E T T F Q T V S W H F C I A L G Y T N S C L N P V L Y A F L D E N F K R C F R E F C I P T S S N I E Q Q N S T R I R Q N T R D H P S T A N T V D R T N H Q L E N L E A E T A P L P G S S G T E T S Q V A P A

Affinity tags

Rho1D4 (C-terminal)
Size (excluding
additional elements)
413 (400) amino acids
45,953 (44,779) Da

Expression system

Sf9 (baculovirus)

Purified via

PureCube Rho1D4 Agarose

Buffer

100 mM NaCl, 20 mM NaH2PO4, pH 7.4

Nanodisc

MSP1E3D1 protein, His-tagged, with POPC lipids

Purity (SDS-PAGE)

>98% (see Fig. 1)

Homogeneity

Size exclusion chromatography

Function

OPRM1 is the most relevant physiological target for most clinically relevant analgesics.  Receptor for endogenous opioids such as beta-endorphin and endomorphin, and  for natural and
synthetic opioids including morphine, heroin, DAMGO, fentanyl, etorphine, buprenorphin and methadone.
Literature references
1. Burford, NT et al. Discovery of positive allosteric modulators and silent allosteric modulators of the µ-opioid receptor. (2013) PNAS 110 (26) 10830-10835

 

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OPRM1 in nanodisc

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OPRM1 protein nanodisc complex (20 ug)

Sales price: 1523,00 €

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