PureCube Alprenolol Agarose

Purification of GPCRs via affinity tags can yield highly pure proteins, but due to the high flexibility of the proteins typically only a fraction of the entire preparation is active. This is often reflected in SEC spectra that show different stages of aggregation. Purifying the same GPCR on a ligand-based affinity column can yield an active, homogeneous preparation.

Whenever a specific ligand for a GPCR of interest is known, we can attempt to couple it to one of our activated agaroses or magnetic beads to yield a purification matrix that is specific to the active conformation of this protein. 

 

One well-known example for such a ligand-based purification is Alprenolol Agarose, which binds to the beta 1 and beta 2 adrenergic receptors. The ligand was coupled via a hydrophilic linker to our  highly crosslinked 7.5% agarose to minimize non-specific binding. This matrix is available off the shelf in mL quantities.

Please contact us for alprenolol magbeads, or for bulk quantities of this matrix.

 

Alprenolol chemical structure

Fig. 1: Chemical structure of (S)-Alprenolol. The PureCube Alprenolol Agarose matrix contains a racemate of the (R) and (S) enantiomers.

 

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Alprenolol Agarose

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PureCube Alprenolol Agarose (1 ml)

Sales price: 650,00 €

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PureCube Alprenolol Agarose (5 ml)

Sales price: 2250,00 €

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