PureCube Ni-IDA Agarose

The His tag is the most widely used affinity tag due to its small size and versatility under native and denaturing conditions, as well as in presence of detergents and many other additives.

Cube Biotech offers high-performance PureCube Ni-IDA Agarose, based on BioWorks Workbeads, for purification of his-tagged proteins. To detect His-tagged proteins in Western Blot experiments, Cube Biotech offers tag-specific antibodies.

 

For increased specificity, we offer a range of different His affinity resins, e.g. carrying the NTA ligand (what's the difference?). Please see our overview page for more information.

 

From Oct 1 to Dec 31, 2018, mention promo code "Cube2018" when placing your order, and save!

 

PureCube Affinity Resins

PureCube His Affinity ResinsPureCube Ni-IDA Agarose

 

Why PureCube Ni-IDA Agarose?

  Binding capacity of up to 50 mg/mL

  Stable under commonly used concentrations of DTT and EDTA

  Can be regenerated for reuse

 

 

Agarose Knowledge Page

 

   

Binding capacity of PureCube Ni-IDA Agarose is comparable to resins from a leading supplier.

Fig. 1: The binding capacity of PureCube Ni-IDA Agarose is comparable to resins from a leading supplier. SDS-PAGE of CAT purified in gravity columns with PureCube Ni-IDA Agarose and Ni resin from Competitor G show elution fractions (E1-E5) with similar protein yields.

 

 

Strong and robust performance

Based on the same agarose matrix with high porosity and physical stability, PureCube Ni-IDA agarose exhibits a protein capacity of 50 mg protein per mL resin, which is competitive with that of products from market-leading providers. Figure 2 shows the SDS-PAGE analysis of 6-His chloramphenicoltransferase (CAT) expressed in E. coli and purified on gravity flow columns filled with PureCUbe Ni-IDA Agarose or Competitor G Ni-Sepharose High Performance resin. The amount of protein drawn down from the columns in 5 elution fractions was comparable for the two affinity resins (CL: cleared lysate; FT: flow-through; W1-2: wash fractions; E1-5: elution fractions). Table 1 compares additional parameters among PureCube Ni-NTA Agarose and two equivalent market-leading resins.

 

 

      Cube Biotech   Competitor G   Competitor S  
  Particle size   32-60 µm   average 90 µm   45-160 µm  
 

Metal ion capacity
(Cu, Ni)

  >25 µmol/mL   >30 µmol/mL   6-18 µmol/mL  
  Binding capacity   50 mg/mL   > 40 mg/ml   15 mg/mL  
  pH stability (processing)   3.0-12.0   3.0-13.0   No information  
 

Recommended flow rate

  0.5-2.0 mL/min  (6.0 mL/min poss.)   1.0 mL/min   1.0 mL/min  
  DTT stability   10 mM   No information   5 mM  
  EDTA stability   1.0 mM   No information   Not recommended  

Table 1. Technical and performance parameters of 3 Ni-IDA agarose resins.

 

 

 

Top-quality materials, competitive performance

PureCube Ni-IDA Agarose is produced with a highly cross-linked agarose which is physically very stable and suitable for purification processes under low pressure (bulk purifications as well as packed in chromatography columns). Additionally, the high porosity and uniform size of the matrix allows for optimal protein interaction and high reproducibility between individual purification runs. Table 1 summarizes technical and performance parameters of PureCube Ni-IDA Agarose and 2 equivalent, market-leading resins.

 

 

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Ni-IDA Agarose

PureCube Ni-IDA Agarose
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PureCube Ni-IDA Agarose (10 ml)

Sales price: 67,00 €

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PureCube Ni-IDA Agarose
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PureCube Ni-IDA Agarose (50 ml)

Sales price: 269,00 €

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PureCube Ni-IDA Agarose
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PureCube Ni-IDA Agarose (250 ml)

Sales price: 1168,00 €

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