PureCube Activated Agaroses XL

 

Affinity chromatography is typically done with agarose beads with particle sizes of 40-150 µm. This material is very well suited for gravity flow and FPLC applications.

Cube Biotech offers PureCube and PureCube 100 agaroses with a large variety of modifications that cover most applications.

 

However, for specialized experiments, agaroses with exceptionally large bead sizes (300-500 µm in diameter) can be useful. For example, large beads settle fast by gravity and are therefore useful for automated purification procedures. Please note that due to the significantly different surface:volume ratio, binding capacity is usually lower than for standard sized beads.

 

Activated Agaroses can be used to couple molecules of your choice in your laboratory. Our careful in-house production provides low lot-to-lot variation, making activated agaroses also suitable for commercial production.

 

 

PureCube Activated Agaroses XL provide:

  Pre-activated matrices for coupling to proteins, peptides, or antibodies

  Easy to follow protocols and expert technical support

  Highly reproducible synthesis in our own laboratories

  High throughput protein purification: Large beads settle fast in 96 well plates

  Compatible with the use of crude lysates

 

 

Activated Agaroses XL are available on request. Please contact us to learn more.

 

 

PureCube Affinity Resins

PureCube Activated ResinsPureCube Extra-large Affinity Resins

Learn more about Agarose Resins

 

Coupling mechanism

 

  Coupling Chemistry   PureCube Activated Agarose XL  

Covalent, via amine groups

  EDC/NHS   Carboxy Agarose XL  

Covalent, via amine groups

Easy coupling of antibodies

 

direct

Fast and easy protocol

  NHS-activated Agarose XL  
Covalent, via cysteine groups   direct   Maleimide-activated Agarose XL  

Coupling of thermostable proteins and small molecules

via amine and thiol

  direct  

Epoxy-activated Agarose XL

 
Covalent, via carboxy groups   direct  

Amine-activated Agarose XL