PureCube Ni-NTA Agarose XL

Affinity chromatography is typically done with agarose beads with particle sizes of 40-150 µm. This material is very well suited for gravity flow and FPLC applications. Cube Biotech offers PureCube and PureCube 100 agaroses with a large variety of modifications that cover most applications.

 

However, for specialized experiments, agaroses with exceptionally large bead sizes (300-500 µm in diameter) can be useful. For example, large beads settle fast by gravity and are therefore useful for automated purification procedures. Please note that due to the significantly different surface:volume ratio, binding capacity is usually lower than for standard sized beads.

 

The polyhistidine tag is the most widely used affinity tag due to its small size, low immunogenicity, and versatility under native and denaturing conditions, as well as in presence of detergents and many other additives. Taking advantage of the affinity of transition metal ions for the imidazole ring of histidine, immobilized metal affinity chromatography (IMAC) is used to purify his-tagged proteins. 

Cube Biotech offers Ni-NTA Agarose XL. For loading with other transition metals, NTA Agarose XL is available on request.

 

PureCube Agarose Affinity Resins

PureCube XL Agarose Affinity Resins

 

 

PureCube Ni-NTA Agarose XL beads provide:

  Automated protein purification: Large beads settle easily in 96 well plates

  Purification of His-tagged proteins

  binding capacity slightly lower than standard matrices (<20 mg/mL)

  Careful in-house production with high lot-to-lot reproducibility

 

Learn more about Agarose Affinity Resins

 

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Ni-NTA Agarose XL

PureCube Ni-NTA Agarose XL
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PureCube Ni-NTA Agarose XL (10 ml)

Sales price: 129,00 €

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PureCube Ni-NTA Agarose XL
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PureCube Ni-NTA Agarose XL (50 ml)

Sales price: 515,00 €

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PureCube Ni-NTA Agarose XL
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PureCube Ni-NTA Agarose XL (250 ml)

Sales price: 2223,00 €

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