PureCube Thiol Activated Agarose

PureCube Thiol Activated Agarose carries a dipyridyl disulphide functionality. Proteins can bind covalently via free cysteines, forming a disulfide bond, and releasing a 2-thiopyridone group. Because  of  the  nature  of  the  disulfide  bond,  the  coupled  proteins  can  also  be  eluted  using reducing agents such as DTT or beta-mercaptoethanol.

 

Different thiol containing proteins can be eluted by using a combination or concentration gradient of weaker (e.g. L-cysteine, reduced glutathione) and stronger (DTT or mercaptoethanol) reducing agents. Thiol Activated Agarose requires 2,2‘-dipyridyl disulphide for activation, which is included in the product.

 

For irreversible coupling of thiol groups, see Maleimide Activated Agarose.

 

PureCube Agarose Affinity Resins

PureCube Activated Agarose Resins

 

 


Please note that Thiol Activated MagBeads and Thiol Activated Agarose have been discontinued effective December 31, 2017. We apologize for any inconvenience this may cause.

 

Learn more about Agarose Resins