PureCube Cu-NTA Agarose

Immobilized metal affinity chromatography, or IMAC, has been employed for many applications making use of its versatile interaction with aromatic nitrogens and phosphates. To this end, NTA matrices have been loaded with a broad range of transition metals, as optimized for the individual interaction.

Cu-NTA Agarose can be used for the purification of his-tagged proteins. It provides high yields of protein, at lower specificity than e.g. Ni- or Co-IMAC. Cu-NTA Agarose has also been used for the enrichment of copper-binding proteins, in particular with plant extracts (1).

 

 

PureCube Affinity Resins

PureCube Alternative IMAC Affinity ResinsPureCube Cu-NTA Agarose

 

 

PureCube Cu-NTA Agarose provides:

  Ready-to-use matrix

  Enrichment of copper-binding proteins

  Purification of his-tagged proteins with different specificity

  Cu-NTA MagBeads also available

 

 

 

 

 

PureCube IMAC Products

Agarose Knowledge Page

Fig. 3: Affinity and specificity of metal ions commonly used for IMAC. Loading an IDA or NTA resin with different metal ions can adjust the affinity and specificity of the resin to optimize the purity and yield of a purified protein.

Fig. 1: Affinity and specificity of metal ions commonly used for IMAC.
Loading an IMAC resin with different metal ions can adjust the affinity and specificity to optimize the purity and yield of a purified protein.
 

Different metal ions confer different binding affinity and specificity

Loading different metal ions to a resin results in differing affinity and specificity for a his-tagged protein. Generally, cobalt exhibits the higest binding specificity of commonly used IMAC metal ions, leading to relatively low yields but high purity. Copper, at the other end of the spectrum, has a high affinity leading to high yields but unspecific binding. In searching for the optimal resin to purify a protein, it is recommended to explore different chelating ligands (IDA or NTA) and different metal ions.


Literature references

1.
Kung. C.C. et al. Proteomic survey of copper-binding proteins in Arabidopsis roots by immobilized metal affinity chromatography and mass spectrometry. Proteomics (2006), 6(9)2746-58.

 

Select Country
Results 1 - 3 of 3

Cu-NTA Agarose

Cu-NTA Agarose
Current Stock Level

PureCube Cu-NTA Agarose (10 ml)

Sales price: 103,00 €

Product details

Cu-NTA Agarose
Current Stock Level

PureCube Cu-NTA Agarose (50 ml)

Sales price: 409,00 €

Product details

Cu-NTA Agarose
Current Stock Level

PureCube Cu-NTA Agarose (250 ml)

Sales price: 1775,00 €

Product details