PureCube Fe-NTA Agarose

Immobilized metal affinity chromatography, or IMAC, has been employed for many applications making use of its versatile interaction with aromatic nitrogens and phosphates. To this end, NTA matrices have been loaded with a broad range of transition metals, as optimized for the individual interaction.

Fe-NTA Agarose is often used for the purification of phosphorylated proteins. Alternative matrices are Al-, Ga- or Zr-NTA.

 

PureCube Affinity Resins

PureCube Alternative IMAC Affinity ResinsPureCube Fe-NTA Agarose

 

PureCube Fe-NTA Agarose provides:

  Ready-to-use matrix

  Purification of phosphorylated proteins

  Enrichment of iron-binding proteins

  Fe-NTA MagBeads also available

 

PureCube IMAC Products

Agarose Knowledge Page

 

Purification of phosphorylated proteins

IMAC methods, in particular Fe-NTA or Ga-NTA, have been widely used to enrich phosphoproteins and phosphopeptides as part of the sample preparation for mass spectrometry (1,3,4). Depending on the sample, and the kind of phosphorylated proteins to be analyzed, also other transition metals, such as zirconium or aluminium have been loaded on NTA or IDA matrices for enrichment (5). Also, magnetic beads have been useful for this kind of sample preparation (2).

 

Literature references

1.
Albuquerque, C.P. et al. A multidimensional chromatography technology for in-depth phosphoproteome analysis. Mol Cell Proteomics (2008), 7(7), 1389-1396.
2.
Herskowitz, J., et al. Phosphoproteomic analysis reveals site-specific changes in GFAP and NDGR2 phosphorylation in frontotemporal lobar degeneration. J. Proteome Res (2010), 9(12):6368-6379.
3.
Yu, P. et al. Global analysis of neuronal phosphoproteome regulation by chondroitin sulfate proteoglycans. PLoS One (2013), 8,3, e59285.
4.
Aryal, U.K. et al. Optimization of immobilized Gallium (III) ion affinity chromatography for selective binding and recovery of phosphopeptides from protein digests. Journal of Biomolecular Techniques (2008), 19:296-310.
5.
Block et. al. Immobilized-metal affinity chromatography (IMAC) a review. Methods Enzymol. (2009), 463:439-73.

 

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Fe-NTA Agarose

Fe-NTA Agarose
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PureCube Fe-NTA Agarose (10 ml)

Sales price: 100,00 €

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Fe-NTA Agarose
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PureCube Fe-NTA Agarose (50 ml)

Sales price: 397,00 €

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Fe-NTA Agarose
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PureCube Fe-NTA Agarose (250 ml)

Sales price: 1723,00 €

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