Rho1D4 & High-purity detergents - the complete solution for membrane proteins

 

 


 

Rho1D4: The perfect purification tag

For almost two decades, the 9-amino acid Rho1D4 affinity tag has been used with tremendous success. With a protein sequence derived from bovine rhodopsin, it enables purification of membrane proteins to high yields and purity on antibody-based matrices.

 

Cube Biotech offers the entire Rho1D4 system for optimal performance:

- Purification matrices based on agarose or magnetic agarose, equipped with the Rho1D4 antibody

- Rho1D4 eluent peptide

- Rho1D4 antibody for detection in Western Blots

- Dedicated protocols and "getting started" guides

 

 

                 Rho1D4 starter sets offer a 50% discount!
  Get started with Rho1D4 in four easy steps.
  Never heard of Rho1D4?

 

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Fig. 1: Purification of chemokine receptor 4 (CXCR4) using PureCube Rho1D4 Agarose. Total E.coli lysate (TL) was solubilized with Fos-Choline-14 and the soluble fraction (SF) was incubated on an immunoaffinity column loaded with rho1D4 antibody. Wash fractions (W1-W3) show no detectable loss of target protein. Concentration of eluted CXCR4 in elution fractions (E1-E4) ranged 0.6-1.0 mg/mL as determined spectrophotometrically.
 

 

High-purity Detergents

Unless you have reconstituted your membrane protein into nanodiscs or similar structures, detergents are required in every project. Careful choice of detergents can be crucial for success with membrane protein projects.

 

Cube Biotech offers a wide range of high-purity detergents to ensure you'll find the one that provides conditions where your protein is well-solubilized and active. To ensure purity and freshness, our detergents are provided in convenient aliquots.

 

Screening sets are perfect tools for quick assessment of optimal detergent type and concentration:

    

Detergent Screening Set Classic:

1 g each of

Decylmaltoside

Dodecylmaltoside

Octylglucoside

Octylthioglucoside

LDAO

 

Detergent Screening Set Phosphocholines:

1 g each of

Fos-12

Fos-14

Fos-16

  Download protocol Download our dedicated detergent screening protocol.

 


 


Questions? Our team of chemists and biologists has decades of experience on membrane protein biochemistry. We provide helpful tips to support your research, and also take on membrane protein service projects.

 

        Contact us today to discuss your project!