Cube Protocols & Datasheets

Our protocols for protein expression, purification and buffer optimization are constantly revised and updated. They are available for free download.


Protocols

Protein purification and detection protocols

CubicPhase Crystallization Protocol

General protocols

Nanodisc protocols

Cell-free protein expression protocol - Standard

Cell-free protein expression protocol - For T7 Lysates

 

Datasheets

 

 

 

 

   

General Protocols

Screening for optimal membrane protein expression conditions using E. coli expression systems

Screening detergents for optimal solubilization and purification of membrane proteins

 

 

 

 

Protocols for protein purification and detection

 

 

His affinity

For PureCube 100 INDIGO Agarose

PureCube 100 Ni-NTA Agarose

PureCube 100 Co-NTA Agarose,    PureCube Ni-NTA Agarose,

PureCube Co-NTA Agarose,

PureCube Ni-IDA Agarose

   GST affinity   Strep affinity    Rho1D4  affinity   Activated

Purification using

Affinity Agarose
 

 

native    denaturing

  native   native   native  

Amine

Carboxy

Epoxy

Maleimide

NHS

Thiol

Purification using 1-step Batch Midi Plus Columns

  native    denaturing   native   native   native  

Carboxy

NHS

Purification using 1-step Batch Mini Columns   native     denaturing   native   native   native   -
Purification using MagBeads   native     denaturing   native   native   native  

Amine

Carboxy

Epoxy

Maleimide

NHS

Thiol

Regeneration

 

Cleaning and Regeneration

  Cleaning and Regeneration   Cleaning and Regeneration   Cleaning and Regeneration   -
Special applications  
 
Nickel loading of IMAC Agarose
Al/Co/Cu/Fe/Zn loading of IMAC Agarose

 

Nickel loading of IMAC MagBeads
AL/Co/Cu/Fe/ZN loading of IMAC MagBeads

 

  Pulldown using MagBeads            
Western Blot detection   Detection   Detection   -   Detection   -

 


 

Nanodisc protocols

   

 

Membrane scaffold protein

 

 

MSP1D1

His-tagged

 

MSP1D1

 

MSP1D1-

deltaH5

His-tagged

 

MSP1D1-

deltaH5

 

MSP1E3D1

His-tagged

 

 

Reconstitution of detergent- solubilized membrane proteins using MSPs

UPDATED 01/16

 

DMPC

 

POPC

 

DMPC

 

POPC

 

DMPC

 

POPC

 

DMPC

 

POPC

 

 

DMPC

 

POPC

 

 

 

Nanodisc assembly for cell-free expression reactions - using Nanodisc Assembly Kits

UPDATED 02/16


 

 

DMPC

 

POPC

 

DMPC

 

POPC

 

DMPC

 

POPC

 

DMPC

 

POPC

 

DMPC

 

POPC

 

 

 


 

Datasheets

His Affinity Agarose & MagBeads   Affinity and Activated Agaroses & MagBeads  

Accessories, Nanodiscs & Columns

Ni-IDA   Rho1D4  
Antibodies

PureCube Ni-IDA Agarose

  PureCube Rho1D4 Agarose  

PentaHis antibody

PureCube Ni-IDA Agarose XL

  PureCube Rho1D4 MagBeads   GST antibody

PureCube Ni-IDA MagBeads

  PureCube Rho1D4 Cartridges   Rho1D4 antibody

PureCube Ni-IDA Cartridges

       
    Glutathione  

Eluent peptides

Ni-NTA

 

PureCube Glutathione Agarose

 

Rho1D4 peptide

 

PureCube Ni-NTA Agarose

PureCube Glutathione Agarose XL

Columns to fill

PureCube 100 Ni-NTA Agarose

PureCube Glutathione MagBeads

PureCube 1-step batch Midi Plus

PureCube Ni-NTA Cartridges

 

PureCube Glutathione Cartridges

  PureCube 1-step batch Mini

PureCube 100 Ni-NTA Cartridges

      PureCube Cartridge, 1 mL

PureCube Ni-NTA Agarose XL

 

StrepTactin

  PureCube Cartridge, 5 mL

PureCube Ni-NTA MagBeads

 

PureCube HiCap StrepTactin Agarose

   

 

 

PureCube HiCap StrepTactin MagBeads

  Active GPCRs
Co-NTA   PureCube HiCap StrepTactin Cartridges   GPBAR1/TGR5

PureCube Co-NTA Agarose

      OPRM1 in nanodisc

PureCube 100 Co-NTA Agarose

      GLP1R

PureCube Co-NTA Cartridges

       

PureCube 100 Co-NTA Cartridge

 

 

   

PureCube Co-NTA Agarose XL

 

Activated Matrices

  Nanodisc scaffold proteins

PureCube Co-NTA MagBeads

  PureCube Amine Activated Agarose   MSP1D1-His
    PureCube Amine Activated MagBeads   MSP1E3D1-His

INDIGO His affinity

  PureCube Carboxy Agarose   MSP1D1 deltaH5-His

PureCube 100 INDIGO Ni-Agarose

  Pure Cube Carboxy MagBeads   MSP2N2-His

PureCube 100 INDIGO Ni-Cartridge

  PureCube Epoxy Activated Agarose   mouse MSP1D1-His

PureCube INDIGO Ni-MagBeads

  PureCube Epoxy Activated MagBeads  

mouse MSP1E3D1-His

    PureCube Maleimide Activated Agarose  

rat MSP1D1-His

Unloaded IDA and NTA   PureCube Maleimide Activated MagBeads  

rat MSP1E3D1-His

PureCube IDA Agarose

  PureCube NHS Activated Agarose    

PureCube IDA MagBeads

  PureCube NHS Activated MagBeads    

PureCube NTA Agarose

  PureCube Thiol Activated Agarose   Nanodisc assembly kits

PureCube 100 NTA Agarose

  PureCube Thiol Activated MagBeads   MSP1D1-His_DMPC

PureCube NTA MagBeads

      MSP1D1-His_POPC
    Ion Exchange Matrices   MSP1D1 deltaH5-His_DMPC
   

PureCube CM Agarose

  MSP1D1 deltaH5-His_POPC

Other NTA Matrices

  PureCube DEAE Agarose   MSP1E3D1-His_DMPC
Cu-NTA Agarose   PureCube Q Agarose   MSP1E3D1-His_POPC
Cu-NTA MagBeads   PureCube SP Agarose    
Fe-NTA Agarose       Ready-to-use Buffers
Fe-NTA MagBeads   Hydrophobic Interaction Matrices   8 M Urea
Al-NTA Agarose   PureCube Butyl Agarose   0.5 M EDTA
Al-NTA MagBeads   PureCube Phenyl Agarose   10X PBS
Zn-NTA Agarose   PureCube Octyl Agarose   50X TAE
Zn-NTA MagBeads       10X TE
    Crystallization   10X TBE
    CubeCrystal Plates   10X TBS
         
         

 

 

StrepTactin® and Strep-tag® are trademarks of IBA GmbH. PureCube StrepTactin Agarose is manufactured by IBA GmbH under German Patent Application No. 42 37 113.9 entitled "Fusion peptides with binding activity for streptavidin"