Cube Protocols & Datasheets

Our protocols for protein expression, purification and buffer optimization are constantly revised and updated. They are available for free download.


Protocols

Protein purification and detection protocols

CubicPhase Crystallization Protocol

General protocols

Nanodisc protocols

Cell-free protein expression protocol - Standard

Cell-free protein expression protocol - For T7 Lysates

 

Datasheets

 

 

 

   

General Protocols

Screening for optimal membrane protein expression conditions using E. coli expression systems

Screening detergents for optimal solubilization and purification of membrane proteins

 

 

 

 

Protocols for protein purification and detection

 

 

His affinity

For PureCube 100 INDIGO Agarose

PureCube 100 Ni-NTA Agarose

PureCube 100 Co-NTA Agarose,    PureCube Ni-NTA Agarose,

PureCube Co-NTA Agarose,

PureCube Ni-IDA Agarose

   GST affinity   Strep affinity    Rho1D4  affinity   Activated

Purification using

Affinity Agarose
 

 

native    denaturing

  native   native   native  

Amine

Carboxy

Epoxy

Maleimide

NHS

Thiol

Purification using 1-step Batch Midi Plus Columns

  native    denaturing   native   native   native  

Carboxy

NHS

Purification using 1-step Batch Mini Columns   native     denaturing   native   native   native   -
Purification using MagBeads   native     denaturing   native   native   native  

Amine

Carboxy

Epoxy

Maleimide

NHS

Thiol

Regeneration

 

Cleaning and Regeneration

  Cleaning and Regeneration   Cleaning and Regeneration   Cleaning and Regeneration   -
Special applications  
 
Nickel loading of IMAC Agarose
Al/Co/Cu/Fe/Zn loading of IMAC Agarose

 

Nickel loading of IMAC MagBeads
AL/Co/Cu/Fe/ZN loading of IMAC MagBeads

 

  Pulldown using MagBeads            
Western Blot detection   Detection   Detection   -   Detection   -

 


 

Nanodisc protocols

   

 

Membrane scaffold protein

 

 

MSP1D1

His-tagged

 

MSP1D1

 

MSP1D1-

deltaH5

His-tagged

 

MSP1D1-

deltaH5

 

MSP1E3D1

His-tagged

 

 

Reconstitution of detergent- solubilized membrane proteins using MSPs

UPDATED 01/16

 

DMPC

 

POPC

 

DMPC

 

POPC

 

DMPC

 

POPC

 

DMPC

 

POPC

 

 

DMPC

 

POPC

 

 

 

Nanodisc assembly for cell-free expression reactions - using Nanodisc Assembly Kits

UPDATED 02/16


 

 

DMPC

 

POPC

 

DMPC

 

POPC

 

DMPC

 

POPC

 

DMPC

 

POPC

 

DMPC

 

POPC

 

 

 


 

Datasheets

 

His Affinity Agaroses   His Affinity MagBeads   His Affinity Cartridges
Ni-IDA   Ni-IDA   Ni-IDA

PureCube Ni-IDA Agarose

 

PureCube Ni-IDA MagBeads

 

PureCube Ni-IDA Cartridge

PureCube Ni-IDA Agarose XL

      PureCube Compact Cartridge Ni-IDA
       
Ni-NTA   Ni-NTA   Ni-NTA

PureCube Ni-NTA Agarose

 

PureCube Ni-NTA MagBeads

 

PureCube Ni-NTA Cartridge

PureCube 100 Ni-NTA Agarose

      PureCube Compact Cartridge Ni-NTA

PureCube Ni-NTA Agarose XL

     

PureCube 100 Ni-NTA Cartridge

        PureCube 100 Compact Cartridge Ni-NTA
         
Co-NTA   Co-NTA   Co-NTA

PureCube Co-NTA Agarose

 

PureCube Co-NTA MagBeads

 

PureCube Co-NTA Cartridge

PureCube 100 Co-NTA Agarose

      PureCube Compact Cartridge Co-NTA

PureCube Co-NTA Agarose XL

     

PureCube 100 Co-NTA Cartridge

        PureCube 100 Compact Cartridge Co-NTA
         

INDIGO His affinity

 

INDIGO His affinity

 

INDIGO His affinity

PureCube 100 INDIGO Ni-Agarose

 

PureCube INDIGO Ni-MagBeads

 

PureCube 100 INDIGO Ni-Cartridge

       

PureCube 100 CompactCartridge Ni-INDIGO

         

Other NTA Matrices

 

Other NTA Matrices

   
Cu-NTA Agarose   Cu-NTA MagBeads    
Fe-NTA Agarose   Fe-NTA MagBeads    
Al-NTA Agarose   Al-NTA MagBeads    
Zn-NTA Agarose   Zn-NTA MagBeads    
       
         
Other Affinity Agaroses   Other Affinity MagBeads   Other Affinity Cartridges
Rho1D4   Rho1D4   Rho1D4
PureCube Rho1D4 Agarose   PureCube Rho1D4 MagBeads   PureCube Rho1D4 Cartridges
        PureCube Compact Cartridge Rho1D4
         
Glutathione   Glutathione   Glutathione

PureCube Glutathione Agarose

 

PureCube Glutathione MagBeads

 

PureCube Glutathione Cartridges

PureCube Glutathione Agarose XL

     

PureCube Compact Cartridge Glutathione

         
StrepTactin   StrepTactin   StrepTactin

PureCube HiCap StrepTactin Agarose

 

PureCube HiCap StrepTactin MagBeads

  PureCube HiCap StrepTactin Cartridges
        PureCube Compact Cartridge HiCap StrepTactin
         
         
         
Activated Agaroses   Activated MagBeads   Other Agaroses & MagBeads
        Unloaded IDA and NTA
PureCube Amine Agarose   PureCube Amine MagBeads  

PureCube IDA Agarose

PureCube Carboxy Agarose   Pure Cube Carboxy MagBeads  

PureCube IDA MagBeads

PureCube Epoxy Agarose   PureCube Epoxy MagBeads  

PureCube NTA Agarose

PureCube Maleimide Agarose   PureCube Maleimide MagBeads  

PureCube 100 NTA Agarose

PureCube NHS Agarose   PureCube NHS MagBeads  

PureCube NTA MagBeads

PureCube Thiol Agarose   PureCube Thiol MagBeads    
        Hydrophobic Interaction Matrices
        PureCube Butyl Agarose
        PureCube Octyl Agarose
        PureCube Phenyl Agarose
         
        Ion Exchange Matrices
       

PureCube CM Agarose

        PureCube DEAE Agarose
        PureCube Q Agarose
        PureCube SP Agarose
         
Nanodisc scaffold proteins   Assembled nanodiscs   Nanodisc assembly kits
Human   Human   Human
MSP1D1 dH5-His   Nanodisc MSP1D1dH5-His_DMPC   MSP1D1 dH5-His_DMPC
MSP1D1 dH5   Nanodisc MSP1D1dH5-His_POPC   MSP1D1 dH5-His_POPC
MSP1D1-His   Nanodisc MSP1D1dH5-His_DMPG   MSP1D1-His_DMPC
MSP1D1   Nanodisc MSP1D1-His_DMPC   MSP1D1-His_POPC
MSP1E3D1-His   Nanodisc MSP1D1-His_POPC   MSP1E3D1-His_DMPC
MSP1E3D1   Nanodisc MSP1D1-His_DMPG   MSP1E3D1-His_POPC
MSP2N2-His   Nanodisc MSP1E3D1-His_DMPC    
MSP2N2   Nanodisc MSP1E3D1-His_POPC    
    Nanodisc MSP1E3D1-His_DMPG    
Mouse/Rat   Nanodisc MSP2N2-His_DMPC    
mouse MSP1D1-His   Nanodisc MSP2N2-His_POPC    

mouse MSP1E3D1-His

  Nanodisc MSP2N2-His_DMPG    

rat MSP1D1-His

  Nanodisc MSP1D1dH5-His_DMPC-BIotinyl-PE    

rat MSP1E3D1-His

  Nanodisc MSP1D1-His_DMPC-BIotinyl-PE    
    Nanodisc MSP1E3D1-His_POPC-BIotinyl-PE    
         
    Mouse/Rat    
    Nanodisc mouse MSP1D1-His_DMPC    
    Nanodisc mouse MSP1D1-His_POPC    
    Nanodisc mouse MSP1E3D1-His_DMPC    
    Nanodisc mouse MSP1E3D1-His_POPC    
    Nanodisc rat MSP1D1-His_DMPC    
    Nanodisc rat MSP1D1-His_POPC    
    Nanodisc rat MSP1E3D1-His_DMPC    
    Nanodisc rat MSPE31D1-His_POPC    
         
Purified Proteins   Protein Crystallization   Accessories
Human GPCRs   CubeCrystal Plates   Columns to fill
GPBAR1/TGR5       PureCube 1-step batch Mini
OPRM1 in nanodisc       PureCube 1-step batch Midi Plus
GLP1R       PureCube Cartridge, 1 mL
NTSR1       PureCube Compact Cartridge, 1 mL
        PureCube Cartridge, 5 mL
Colored membrane proteins   Ready-to-use buffers   PureCube Compact Cartridge, 5 mL
Bacteriorhodopsin   8 M Urea    
Hs Sensory rhodopsin-1   0.5 M EDTA   Antibodies
Hs Sensory rhodopsin-2   10X PBS  

PentaHis antibody

Np Sensory rhodopsin-2   50X TAE   GST antibody
Channelrhodopsin 1   10X TE   Rho1D4 antibody
    10X TBE    
Green fluorescent proteins   10X TBS   Eluent peptides
eGFP-His      

Rho1D4 peptide

GST-eGFP-Rho        
GST-eGFP-DYKDDDDK        
         

 

 

StrepTactin® and Strep-tag® are trademarks of IBA GmbH. PureCube StrepTactin Agarose is manufactured by IBA GmbH under German Patent Application No. 42 37 113.9 entitled "Fusion peptides with binding activity for streptavidin"