SARS CoV-2 spike protein in DIBMA Glycerol
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SARS-CoV-2, more commonly known as the coronavirus, enters the cell by using its surface... more
Product information "SARS CoV-2 spike protein in DIBMA Glycerol"
| Product Type: | Spike protein |
| Protein Sequence: | Full-length |
SARS-CoV-2, more commonly known as the coronavirus, enters the cell by using its surface protein called SPIKE. SPIKE is processed on the cell's surface through the serine protease TMPRSS2 and subsequently binds to the surface receptor ACE2.
The native SPIKE protein is a trimer and is located in the coronavirus membrane. Therefore to obtain pure & native SPIKE the trimer needs to be kept intact. We achieved this in three different ways:
The native SPIKE protein is a trimer and is located in the coronavirus membrane. Therefore to obtain pure & native SPIKE the trimer needs to be kept intact. We achieved this in three different ways:
- MSP nanodiscs, based on MSP proteins
- Detergent Mycelles
- Synthetic nanodiscs, as you can see here
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Figure 1: Data from our lab regarding SPIKE stabilized and purified in synthetic nanodiscs (Dibma Glycerol).A: SDS of SPIKE in synthetic nanodisc.
B: Western blot of Rhot1d4 tagged SPIKE, originated from the SDS Page seen in "A"
C: Native protein Gel of purified and stabilized SPIKE in synthetic nanodisc.
Features
| Alternative names | SPIKE_SARS2 Spike glycoprotein |
| UniProt number | P0DTC2 |
| Protein class | Single span transmembrane protein |
| Organism | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2) |
| Sequence |
|
| Affinity tag | C-terminal Rho1D4 |
| Expression Host | Hek293 Expi cells |
| Size | 1286 amino acids (including Rho1D4 tag and linker) 142114 Da |
| Buffer composition | 20 mM Hepes pH 7.5; 150 mM NaCl, buffer does not contain free DIBMA Glycerol (DIBMA: Diisobutylene-maleic acid) |
| Function | host cell surface receptor binding; fusion of virus membrane with host endosome membrane |
| Purity (SDS-PAGE) | >98% as determined by SDS-PAGE, see Fig. 1 A and B |
| Homogeneity | Native Gel, see Fig. 1 C |
Related links to "SARS CoV-2 spike protein in DIBMA Glycerol"
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