August 2017 New application note on Malaria POC diagnostics

IMAC increases sensitivity in Malaria diagnostics

Immobilized metal affinity chromatography (IMAC) is a popular method for protein purification, particularly for his-tagged recombinant proteins. But IMAC can be used in many other applications. Its high binding capacity towards the malaria biomarker protein HRP2 makes it an excellent enrichment method to increase sensitivity in malaria point-of-care diagnostic assays.
David W. Wright and his team at Vanderbilt University, Nashville, TN, USA have developed a novel device that efficiently combines proven point-of-care lateral flow assays with IMAC magnetic bead enrichment, which is deployed in collaboration with the Macha Research Trust in Zambia. In their work, Cube Biotech's Zn-NTA MagBeads showed highest biomarker protein recovery at lowest cost.
Dibma purification westernblot
Fig. 1: Cube Biotech's Zn-NTA MagBeads provides most efficient HRP2 capture at lowest cost. Comparable volumes of IMAC MagBeads from four different manufacturers were evaluated as described in the application note.
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