Synthetic Nanodisc Screening Kit MAXI (16x2x50 mg)

Overview of all Synthetic polymers that are contained in the MAXI screening Kit
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Which polymer to use for your nanodisc? Note:  We also offer our Synthetic... more
Product information "Synthetic Nanodisc Screening Kit MAXI (16x2x50 mg)"

Which polymer to use for your nanodisc?

SMA or DIBMA
Note: We also offer our Synthetic Nanodisc Screening kit MINI. It covers DIBMA and SMA, but not AASTY or Amphipols.
Finding the best performing snythetic nanodisc polymer for your respective nanodisc can be tricky. Especially if you have never worked with your current membrane protein of interest before. For these cases we at Cube Biotech compiled our most prominent SMALP, DIBMA and AASTY products into this practical Synthetic Nanodisc Screening Set MAXI for your convenience!
All 16 products are buffered in HEPES to provide maximal comparability between the individual screening assays. Which 16 products are include in the Kit precisely can be found in the product's description.

Figure 1 shows the necessity for a screening assay instead of using a random polymer product.
Screening Assay of different synthetic polymers for membrane protein solubilization Figure 1: Performance of membrane protein solubilization under different synthetic polymers. All samples contained the same membrane protein of interest. Furthermore, two different polymer concentrations were tested as well. 2.5% and 0.5%.

Included SMALP Products

Feature SMALP
140
SMALP
140-I
SMALP
200
SMALP
300
Polymer styrene-to-maleic-anhydride ratio [n:m] 1.4:1 1.4:1 2:1 3:1
Molecular weight 5,000 Da 5,000 Da 6,500 Da 10,000 Da
Form Lyophilized powder Lyophilized powder Lyophilized powder Lyophilized powder
pH, after solving 7.5 7.5 7.5 7.5
Divalent cationic tolerance < 5 mM < 100 mM < 5 mM < 5 mM
Color Amber Brownish Yellowish Colorless
Odor Odorless Odorless Odorless Odorless
Other noteworthy feature   is imidized with DMAPA Most commonly used SMA  

Included DIBMA Products

15701 g/Mol
Feature DIBMA 10 DIBMA 12 DIBMA
Glycerol
DIBMA
Glucosamine
Glyco-DIBMA
Formula Weight 10,000 Da 12,000 Da 10,000 Da 10,000 Da 15700 Da
Solubility > 10 % in H2O
Absorbance at 280 nm < 0.3 (1 % solution)
Mg2+ Tolerance 25 mM 25 mM >50 mM >50 mM 25 mM
Ca2+ Tolerance starts to precipitate 50 mM starts to precipitate at 50 mM >50 mM >50 mM starts to precipitate at 50 mM

Included AASTY Products

Feature AASTY 6-45 AASTY 6-50 AASTY 6-55 AASTY 11-45 AASTY 11-50 AASTY 11-55
Molecular weigth 5-6 kDa 5-6 kDa 5-6 kDa 10-11 kDa 10-11 kDa 10-11 kDa
pH after solving 7.5 7.5 7.5 7.5 7.5 7.5
Divalent cationic tolerance 6 mM 6 mM 6 mM 6 mM 6 mM 6 mM
Acrylic Acid to Styrene Ratio 45% to 55% 50% to 50% 55% to 45% 45% to 55% 50% to 50% 55% to 45%

Ultrasolute™ Amphipol Products

Feature Ultrasolute™ 17
Coming soon
Ultrasolute™ 18
pH after solving 7.5 ± 0.3 7.5 ± 0.3
Solubility 10% (H2O) 10% (H2O)
Molecules that make up the polymer
1:1 ratio
2-Cyclohexyl-ethyl acrylamide / acrylic acid 2-cyclohexyl-ethyl acrylamide (COA) / acrylic acid (AA)

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