Synthetic Nanodisc Screening Kit MINI, (8x2x50 mg)

Synthetic Nanodisc Screening Kit MINI, (8x2x50 mg)
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  • 18290
Note:  On October 1st 2021 all SMALP products in this kit were renamed. Nothing... more
Product information "Synthetic Nanodisc Screening Kit MINI, (8x2x50 mg)"
SMA or DIBMA
Note: On October 1st 2021 all SMALP products in this kit were renamed. Nothing else has changed. More on this renaming can be found here:
Finding the best performing synthetic nanodisc polymer for your respective nanodisc can be tricky. Especially if you have never worked with your current membrane protein of interest before. For these cases we at Cube Biotech compiled our most prominent SMALP and DIBMA products into this practical Synthetic Nanodisc Screening Set for your convenience!
All 8 products are buffered in HEPES to provide maximal comparability between the individual screening assays. Which 8 products are include in the Kit precisely can be found in the product's description.

Figure 1 shows the necessity for a SMALP screen instead of using a random SMALP product. Note that the detergent LMNG may provide the mighest protein yield, but it has to be added to the purified protein constantly and it might interfere with the SPIKE protein's function.
SPIKE COV-2 in SMA Figure 1: Example of a SMALP screening assay. Different SMALPs compared in after solubilisation & purification of Rho1-tagged SPIKE protein of coronavirus.

Included SMALP Products

Feature SMALP
140
SMALP
140-I
SMALP
200
SMALP
300
Polymer styrene-to-maleic-anhydride ratio [n:m] 1.4:1 1.4:1 2:1 3:1
Molecular weight 5,000 Da 5,000 Da 6,500 Da 10,000 Da
Form Lyophilized powder Lyophilized powder Lyophilized powder Lyophilized powder
pH, after solving 7.5 7.5 7.5 7.5
Divalent cationic tolerance < 5 mM < 100 mM < 5 mM < 5 mM
Color Amber Brownish Yellowish Colorless
Odor Odorless Odorless Odorless Odorless
Other noteworthy feature   is imidized with DMAPA Most commonly used SMA  

Included DIBMA Products

Feature DIBMA 10 DIBMA 12 DIBMA
Glycerol
DIBMA
Glucosamine
Formula Weight 10,000 Da 12,000 Da 10,000 Da 10,000 Da
Solubility > 10 % in H2O
Absorbance at 280 nm < 0.3 (1 % solution)
Mg2+ Tolerance 25 mM 25 mM >50 mM >50 mM
Ca2+ Tolerance starts to precipitate 50 mM starts to precipitate at 50 mM >50 mM >50 mM

PDF Downloads

Safety Data Sheets (SDS) - DIBMA


Technical Data Sheets - DIBMA

Safety Data Sheets (SDS) - SMA


Technical Data Sheets - SMA


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