SMALP Screening Set (4x3x50 mg)

SMALP Screening Set (4x3x50 mg)
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Note:  On October 1st 2021 all SMALP products in this kit were renamed. Nothing else... more
Product information "SMALP Screening Set (4x3x50 mg)"
Note: On October 1st 2021 all SMALP products in this kit were renamed. Nothing else has changed. More on this renaming can be found here:
Membrane protein and their composition as as versatile as the whole cell membrane itself. For this reason it is sadly impossible to predict with a 100% accuracy which SMALP product is suited best for the solubilization & subsequent of your membrane protein of interest.
For this reason we offer this SMALP Screening Kit that consists of our four individual SMAs. This way you have a convenient way to screen for the best SMA for your protein before scaling up your assay.
In case you would like to extend your polymer screening beyond the reaches of SMA we offer our Synthetic Nanodisc Screening Kit, that covers both SMA and DIBMA.

Figure 1 shows the necessity for a SMALP screen instead of using a random SMALP product. Note that the detergent LMNG may provide the mighest protein yield, but it has to be added to the purified protein constantly and it might interfere with the SPIKE protein's function.
SPIKE COV-2 in SMA Figure 1: Example of a SMALP screening assay. Different SMALPs compared in after solubilisation & purification of Rho1-tagged SPIKE protein of coronavirus.

Our SMALP Screening Set consits off the following four individual products:

Feature SMALP
140
SMALP
140-I
SMALP
200
SMALP
300
Polymer styrene-to-maleic-anhydride ratio [n:m] 1.4:1 1.4:1 2:1 3:1
Molecular weight 5,000 Da 5,000 Da 6,500 Da 10,000 Da
Form Lyophilized powder Lyophilized powder Lyophilized powder Lyophilized powder
pH, after solving 7.5 7.5 7.5 7.5
Divalent cationic tolerance < 5 mM < 100 mM < 5 mM < 5 mM
Color Amber Brownish Yellowish Colorless
Odor Odorless Odorless Odorless Odorless
Other noteworthy feature   is imidized with DMAPA Most commonly used SMA  
Western Blot using SMALPS Figure 2: Western Blot of a purified membrane protein after stabilization through SMA.
Reproduced with permission from Ms S. Nestorow, University of Birmingham.

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