Ultrasolute™ Amphipol 18 HEPES

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Ultrasolute TM Amphipol 18 from Cube Biotech is a highly purified copolymer of cyclooctyl... more
Product information "Ultrasolute™ Amphipol 18 HEPES"
UltrasoluteTM Amphipol 18 from Cube Biotech is a highly purified copolymer of cyclooctyl acrylamide (COA) and acrylic acid (AA), with a molecular weight (Mw) of ~7.300. After dissolving, the copolymer is in a concentration from 1.0 to 5.0%.

UltrasoluteTM Amphipol 18 from Cube Biotech is a highly purified copolymer of cyclooctyl acrylamide and acrylic acid, with a molecular weight (Mw) of ~7.300. After dissolving, the copolymer is in a concentration from 1.0 to 5.0%. Copolymers provide a hydrophobic surface facing the membrane phospholipids and a hydrophilic surface at the hydrophilic outside. This setup makes nanodiscs highly soluble in aqueous solutions and allows for the solubilization of membrane proteins in the absence of detergents. As a secondary possibility the products can be used to creat 100% artificial nanodiscs. Phospholipids such as dimyristoyl-glycero-phosphocholine (DMPC) or palmitoyl-oleoyl-phosphatidyl-choline (POPC) have been proven to work here in combination with sodium cholate. The complex off Ultrasolute™ Amphipol 18 and membrane protein can be used in many biophysical assays, such as SDS-PAGE, SEC, Western Blot, UV/Vis spectroscopy, and many chromatographic procedures.

Ultrasolute™ Amphipol 18 is part of our Synthetic Nanodisc Screening Kit MAXI. Together with 15 other nanodisc polymers you can screen for the best one for your membrane protein.

Table 1: Features

State Lyophilized Powder,
to be solved with water
Buffer HEPES
pH after solving 7.5
Adsorbance < 0.1 (280 nm, 1% solution)
COA/AA Ratio 1:1
Solubility > 10% (H2O)
Structure Amphipol 18 structureStructure of Ultrasolute Amphipol 18™

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