PureCube IDA agarose resin was developed for IMAC based purification and enrichment methods. Since the affinity of an IDA-metal-ion-complex depends on the loaded metal ion, we offer IDA agarose resin uncoupled to any metal ion for the case that we do not offer your desired IDA + metal ion combination. By providing our coupling protocols, feel free to create any IDA purification resin you desire. PureCube IDA resin cartridges are pre-filled FPLC columns that contain our PureCube IDA agarose. The cartridges were designed for maximum compability with most FPLC hardware. Please check the given features table to confirm their compability with your hardware. For the rare case incompability between our cartridges and your FPLC machinery have a look at our PureCube IDA COMPACT cartridges. They were designed for this kind of situation. Furthermore PureCube IDA can also be purchased magnetic beads / MagBeads.
Features - Regarding the resin.
Usage
For coupling of metal ions for corresponding purification and / or enrichment purposes
Specifity
Varies
Binding capacity
Depends on the loaded ion
Purity
Depends on the loaded ion
Bead Ligand
IDA
Bead size
40 μm
Matrix
7.5% cross-linked agarose
Recommended FPLC flow rates
0.5-2 mL/min
Maximal FPLC flow rate
6 mL/min
pH stability
2-14
Other stabilities
100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
