Custom IMAC products

Immobilized metal affinity chromatography, or IMAC for short has many applications in the field of proteomics. Among these purposes are:

In all cases a IMAC agarose resin or MagBead can be used for His-tag protein purification. Our products dedicated to this purpose you will find here.
Phosphopeptide enrichment is performed with Zr, Al, Fe and Ti IMAC products. You will find our products dedicated to this purpose here.
Other more specialized purposes of IMAC products are performed with Zn and Cu-IMAC products.

However these lists might still not cover your purpose and you desire an IMAC product with a coupled metal ion that cannot be found on the market. For this situation Cube Biotech offers its high quality NTA and IDA resin and MagBeads uncoupled to any metal ion. Choose the metal ion you want to couple. Just follow our simple coupling protocol and create the IMAC resin / MagBead you desire.

Overview of Cube Biotech NTA and IDA products for costumized metal ion coupling.
Not sure what the difference between NTA and IDA is?


	IDA products
IDA agarose (40 µm)	IDA agarose (40 µm) Cartridge	IDA MagBeads (30 µm)
IDA agarose (XL sized)	IDA agarose (40 µm) COMPACT Cartridge	IDA MagBeads (XL sized)
	NTA products
NTA agarose (40 µm)	NTA agarose (40 µm) Cartridge	NTA MagBeads (30 µm)
NTA agarose (100 µm)	NTA agarose (40 µm) COMPACT Cartridge	NTA MagBeads (XL sized)
NTA agarose (XL sized)

Note: If you are want us to load a metal ion of your choice, just contact us for our customized agarose resin and MagBead service.

PureCube NTA Agarose from Cube Biotech was successfully used in the following publications:

Coupled molecule / metal ion	Purified substance	Year	Author
Gallium	Phages	2019	Schönberger N., Braun R., Matys S., Lederer F-L., Lehmann F., Flemming K., Pollmann K.¹
Gallium	Gallium binding Peptides	2021	Schönberger N.²

References:

Schönberger N., et al. Chromatopanning for the identification of gallium binding peptides. Elsevier; Jornal of Chromatography A, Published August 30, 2019
Schönberger N. Identification and characterization of galliumbinding peptides. Dissertation; Faculty of Chemistry and Physics, Technical University Bergakademie Freiberg, Date of Conferment 9th, October 2020