Product information "PureCube 100 INDIGO Ni-Agarose"
Type:
Agarose
Bead size:
100 µm (Agarose)
Affinity :
His-tag
Purpose:
Protein Purification
Ligand:
INDIGO
Coupled Ion:
Ni2+
PureCube 100 INDIGO-Ni Agarose resin is a unique development by Cube Biotech for His-tag protein purifications of the highest quality. Created with the aim in mind to create an agarose resin that can withstand highest concentrations of chelators like EDTA and DTT, which are common in mammalian cell culture buffers. The resulting INDIGO ligands has DTT and EDTA tolerances of 20 mM each. That is highly superior to other competing resins. INDIGO-Ni is also available as Magnetic Beads or as pre-packed in cartridges / columns.
Features - Regarding Purification capabilities.
Feature
Usage
Specific binding and purification of 6x his-tagged proteins
Specifity
Affinity to His-tagged proteins
Binding capacity
>100 mg/mL
Bead Ligand
INDIGO-Ni
Bead size
100 μm
Concentration of resin
50%
Matrix
6% highly cross-linked agarose
Chelator stability
Stable in buffer containing 20 mM DTT and 20 mM EDTA
