PureCube INDIGO-Ni magnetic beads / MagBeads were developped by Cube Biotech for His-tag protein purification. To create magnetic beads of the highest quality that can tolerate highest concentrations of chelators like EDTA and DTT, which are aften used in mammalian cell culture buffers. The resulting INDIGO ligand has EDTA and DTT stabilities of 20 mM each. That is highly superior to other competing magnetic beads like Ni-NTA. INDIGO-Ni is also available as agarose resin beads or as pre-packed in cartridges / columns.
Features - Regarding Purification capabilities.
Feature
Usage
Specific binding and purification of 6x His-tagged proteins
Specifity
Affinity to His-tagged proteins
Binding capacity
>100 mg/mL
Bead Ligand
INDIGO-Ni
Bead size
30 µm
Filling quantity
Delivered as a 25 % suspension
Chelator stability
Stable in buffer containing 20 mM DTT and 20 mM EDTA
pH stability
2-14
Other stabilities
100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
