Product information "PureCube 100 INDIGO Ni-Cartridge"
Type:
Cartridge
Bead size:
100 µm (Agarose)
Affinity :
His-tag
Purpose:
Protein Purification
Ligand:
INDIGO
Coupled Ion:
Ni2+
The PureCube INDIGO-Ni Column / Cartridge is an IMAC chromatography column prepacked with PureCube 100 INDIGO-Ni Ni-IDA Agarose resin beads. You can choose between two sizes, 1 mL bed volume and 5 mL bed volume. Please have a look at the given features table to ensure if the Cartridge / Column is compatible with your FPLC hardware. If there are lacking compatibility we recommed to check out our INDIGO-Ni COMPACT Cartridges , that are covering additional compabilities.
Features - Regarding Purification capabilities.
Feature
Usage
Specific binding and purification of 6x his-tagged proteins
Specifity
Affinity to His-tagged proteins
Binding capacity
>100 mg/mL
Bead Ligand
INDIGO-Ni
Bead size
100 μm
Matrix
6% highly cross-linked agarose
Chelator stability
Stable in buffer containing 20 mM DTT and 20 mM EDTA
ph Stability
2-13
Features - Regarding Cartridge / Column itself.
Feature
PureCube Cartridge 1 mL
PureCube Cartridge 5 mL
Bed Volume
1 mL
5 mL
Dimensions: diameter X length (mm)
6.2 x 50
11 x 80
Body material
Polypropylene
Acrylate
End Plug Material
Polypropylene
Inlet
10-32 UNF female thread
Outlet
10-32 UNF female thread
Max. Flow Rate
6 mL/min
PDF Downloads
*The protocols on how to use the cartridges / columns are dependent on the used machine, therefore we unfortunately cannot provide a specific protocol here.
Related links to "PureCube 100 INDIGO Ni-Cartridge"
