Our PureCube Ni-NTA agarose resins are small beads with a diameter of 40 µm. They are used for the purification of active His tagged proteins from cells. Our year long experience in manufacturing agarose resin lead to the high yield of 80 mg protein per ml resin, which is leading in the market compared to other Ni-NTA suppliers. PureCube Ni-NTA resins are suited for batch spin columns and FPLC. Their small diameter provides them with a great mechanical stability. For higher flow rates we recommend our Ni-NTA agarose beads with 100 µm diameter and for extreme cases our XL sized Ni-NTA beads.
PDF Downloads
Features
Usage
Specific binding and purification of 6x His tagged proteins
Specifity
Affinity to His tagged proteins
Binding capacity
>80 mg/mL
Bead Ligand
Ni-NTA
Bead size
40 μm
Chelator stability
Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity
Delivered as a 50 % suspension
Required equipment
Lysis Buffer
Wash Buffer
Elution Buffer
Ice bath
Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
50 mL centrifuge tube
Micropipettor and Micropipetting tips
Disposable gravity flow columns with capped bottom outlet, 2 ml
pH meter
End-over-end shaker
SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
