- Order number: 74301
We offer our 100 PureCube Ni-NTA agarose resin pre-filled in Columns / Cartridges for FPLC... more
Product information "PureCube 100 Ni-NTA Cartridge"
Type: | Cartridge |
Bead size: | 100 µm (Agarose) |
We offer our 100 PureCube Ni-NTA agarose resin pre-filled in Columns / Cartridges for FPLC based His Tag protein purification. The columns / cartridges are compatible with many FPLC machines like ÄKTAs. Consult the corresponding features table to find out if they are matching your hardware. Alternatively we also offer columns / cartridged with different measurements that we call COMPACT cartridges / columns in case these cartridges here are not compatible with your hardware. Alternatively we offer the same cartridges filled with smaller Ni-NTA beads (40 µm) if protein yield is of more importance than flow rates.
Features - Regarding Purification capabilities.
Feature | |
Usage | Specific binding and purification of 6x his-tagged proteins |
Specifity | Affinity to His-tagged proteins |
Binding capacity | >80 mg/mL |
Bead Ligand | Ni-NTA |
Bead size | 100 μm |
Matrix | 6% highly cross-linked agarose |
Chelator stability | Stable in buffer containing 10 mM DTT and 1 mM EDTA |
ph Stability | 2-14 |
Features - Regarding Cartridge / Column itself.
Feature | PureCube Compact Cartridge 1 mL | PureCube Compact Cartridge 5 mL |
Bed Volume | 1 mL | 5 mL |
Dimensions: diameter X length (mm) | 6.2 x 50 | 11 x 80 |
Body material | Polypropylene | Acrylate |
End Plug Material | Polypropylene | |
Inlet | 10-32 UNF female thread | |
Outlet | 10-32 UNF female thread | |
Max. Flow Rate | 6 mL/min |
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