Product information "PureCube 100 Ni-NTA Cartridge"
Type:
Cartridge
Bead size:
100 µm (Agarose)
We offer our 100 PureCube Ni-NTA agarose resin pre-filled in Columns / Cartridges for FPLC based His Tag protein purification. The columns / cartridges are compatible with many FPLC machines like ÄKTAs. Consult the corresponding features table to find out if they are matching your hardware. Alternatively we also offer columns / cartridged with different measurements that we call COMPACT cartridges / columns in case these cartridges here are not compatible with your hardware. Alternatively we offer the same cartridges filled with smaller Ni-NTA beads (40 µm) if protein yield is of more importance than flow rates.
Features - Regarding Purification capabilities.
Feature
Usage
Specific binding and purification of 6x his-tagged proteins
Specifity
Affinity to His-tagged proteins
Binding capacity
>80 mg/mL
Bead Ligand
Ni-NTA
Bead size
100 μm
Matrix
6% highly cross-linked agarose
Chelator stability
Stable in buffer containing 10 mM DTT and 1 mM EDTA
ph Stability
2-14
Features - Regarding Cartridge / Column itself.
Feature
PureCube Compact Cartridge 1 mL
PureCube Compact Cartridge 5 mL
Bed Volume
1 mL
5 mL
Dimensions: diameter X length (mm)
6.2 x 50
11 x 80
Body material
Polypropylene
Acrylate
End Plug Material
Polypropylene
Inlet
10-32 UNF female thread
Outlet
10-32 UNF female thread
Max. Flow Rate
6 mL/min
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*The protocols on how to use the cartridges / columns are dependent on the used machine, therefore we unfortunately cannot provide a specific protocol here.
