PureCube Phenyl Agarose
- Order number: 39103
Our PureCube Phenyl Agarose has been synthesized for hydrophobic interaction chromatography... more
Product information "PureCube Phenyl Agarose"
| Type: | Agarose |
| Bead size: | 40 µm (Agarose) |
| Affinity : | Hydrophobic Interaction |
| Purpose: | Protein Purification |
Our PureCube Phenyl Agarose has been synthesized for hydrophobic interaction chromatography (HIC) based protein purification assays. Usually high salt concentration achieved are required for protein bindung, while elution is performed with a decreasing salt gradient.
Purifying a novel protein in this way requires testing and screening through of different hydrophobic matrices to find the best suited on for your protein. For this screening purpose we also offer additional HIC agarose resin, namely PureCube Butyl Agarose and PureCube Octyl Agarose.
All three typed of PureCube HIC agarose resin (Butyl, Phenyl and Octyl-) can be purchased together in our practical PureCube HIC Starter Set.
Purifying a novel protein in this way requires testing and screening through of different hydrophobic matrices to find the best suited on for your protein. For this screening purpose we also offer additional HIC agarose resin, namely PureCube Butyl Agarose and PureCube Octyl Agarose.
All three typed of PureCube HIC agarose resin (Butyl, Phenyl and Octyl-) can be purchased together in our practical PureCube HIC Starter Set.
Schematic of a Phenyl groupFeatures
| Usage | Protein Purification using hydrophobic interactions |
| Bead Ligand | Covalently coupled phenyl group |
| Butyl group density | 40 µmol/ml |
| Binding capacity for BSA | 25 mg/ml |
| Binding capacity for Lysozyme | 45 mg/ml |
| Matrix | 7.5% cross-linked agarose |
| Bead size | 40 μm |
| pH stability | 2-13 |
| Filling quantity | Delivered as a 50 % suspension |
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