Product information "PureCube Compact Cartridge Fe-NTA"
Type:
Cartridge
Bead size:
40 µm (Agarose)
Purpose:
Phosphopeptide Enrichment
Ligand:
NTA
Coupled Ion:
Fe2+
Packing your affinity resin into a cartridge takes up time that can be spend more efficiently in the lab. Therefore we pre-packed our PureCube Fe-NTA agarose resin beads into columns for FPLC for your convenience. It is recommended to check if our cartridges / columns are indeed compatible with your FPLC hardware by consulting the given features table. For the case that your hardware is incompatible to our cartridges, check out our other PureCube Fe-NTAcartridges. In addition to agarose resin Cube Biotech also offers PureCube Fe-NTA Magnetic Beads / MagBeads.
Features - Regarding Purification capabilities.
Feature
Usage
Enrichment of phosphopeptides
Specific binding and purification of 6x his-tagged proteins
Specifity
Affinity to His-tagged proteins
Affinity to phosphorylated biomolecules
Matrix
7.5% cross-linked agarose
Bead Ligand
Fe-NTA
Bead size
40 µm
Filling quantity
Delivered as a 50 % suspension
Chelator stability
Stable in buffer containing 10 mM DTT and 1 mM EDTA
pH stability
2-14
Other stabilities
100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
Features - Regarding Cartridge / Column itself.
Feature
PureCube Compact Cartridge 1 mL
PureCube Compact Cartridge 5 mL
Bed Volume
1 mL
5 mL
Dimensions: diameter X length (mm)
8.0 x 35
17 x 35
Body material
Polypropylene
End Plug Material
Polypropylene
Inlet
10-32 UNF female thread
Outlet
10-32 UNF female thread
Max. Flow Rate
6 mL/min
PDF Downloads
Related links to "PureCube Compact Cartridge Fe-NTA"
