Our PureCube Ti-NTA magnetic beads / Magbeads were developed for the enrichment of phosphorylated biomolecules like peptides and proteins. The affinity matrix is composed of 6% cross-linked agarose with an average bead diameter of 30 µm. The very homogeneous setup of the MagBead suspension allows for a high degree of reproducibility between indivudual experiments. The concentration of the magnetic bead suspension is 25%. We recommend this paper by Leutert et al. (2019) for you too the possible applications for Ti-NTA products in the field of phosphopeptide enrichment.
Features - Regarding Purification capabilities.
Feature
Usage
Enrichment of phosphopeptides
Specifity
Affinity to phosphorylated biomolecules
Bead Ligand
Ti-NTA
Bead size
30 µm
Filling quantity
Delivered as a 25 % suspension
Chelator stability
Stable in buffer containing 10 mM DTT and 1 mM EDTA
pH stability
2-14
Other stabilities
100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
