HighSpec Rho1D4 Agarose

Order number: 33103

€1,755.00*

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Description

HighSpec Rho1D4 Agarose resin is a suspension of 40 µm agarose beads, coupled to an anti-Rho1D4 antibody. It is meant for protein purification assays of the highest possible purity, exploiting the specificity of an antibody to its antigen. More on the Rho1D4-tag can be read here.
You can also purchase these agarose beads pre-packed into FPLC-ready cartridges. Additionally, we offer Rho1D4-tag affinity resin with a bead diameter of 400 µm for very viscous cell media and higher FPLC flow rates. Alternatively, Rho1D4 MagBeads are also available, if you prefer this affinity chromatography method.

Additional support products for Rho1D4-tag purification assays are Rho1D4 peptide, an essential compound of the elution buffer, and our Rho1D4 antibody for later western blot detection of your protein.

You are using the Rho1D4-tag for the first time? For this case, we recommend using one of our three Rho1D4 Starter Kits. Each combined one of our Rho1D4-tag purification products + 5 mg of the Rho1D4 elution peptide.
Feature
Usage Specific binding and purification of Rho1D4-tagged proteins
Specificity Affinity to Rho1D4-tagged (TETSQVAPA) proteins
Binding capacity >3 mg/ml
Bead Ligand anti-Rho1D4 antibody
Bead size 100 μm
Matrix 6% cross-linked agarose
Recommended flow rates 0.25 - 1 ml/min
Max. flow rates 6 ml/min
Filling quantity Delivered as a 50 % suspension
Short-term storage In neutral buffer at 4°C
Long-term storage In neutral buffer with 20% ethanol at 4°C
Required equipment
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer (incl. Rho1D4 peptide)
  • Ice bath
  • Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
  • 50 mL centrifuge tube
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  • pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment

Citations

Purified ProteinYearAuthor
Rhodopsin Mutants 2015 Mattle D., Singhal A., Schmid G., Dawson R., Standfuss J.
PRG2 2019 Brosig A., Fuchs J., Ipek F., Kroon C., Schrötter S., Vadhvani M., Polyzou A., Ledderose J., van Diepen M., Holzhütter H-G., Trimbuch T., Gimber N., Schmoranzer J., Lieberam I., Rosenmund C., Spahn C., Scheerer P., Szczepek M., Leondaritis G., Eickholt B.J.
Opsin 2019 Napolitano A., van der Veen A.G., Bunyan M., Borg A., Frith D., Howell S., kjaer S., Beling A., Snijders A.P., Knobeloch K-P., Frickel E-M.
NeoR 2020 Broser M., Spreen A., Konold P.E., Peter E., Adam S., Borin V., Schapiro I., Seifert R., Kennis J.T.M., Sierra Y.A.B., Hegemann P.
Gαs 2021 Swonger Koretz K., McGraw C.E., Stradley S., Elbaradei A., Malstadt N., Robinson A.S.
emST 2021 Bradberry M.M., Mishra S., Zhang Z., Wu L., McKetney J.M., Vestling M.M., Coon J.J., Chapman E.R
Immunoisolation of synpatic vesicles 2022 Weihou G.
2022 Rozenberg A., Kaczmarczyk I., Matziv D., Verock J., Nagata T., Sugiura M., Katayama K., Kawasaki Y., Konno M., Nagasaka Y., Aoyama M., Das I., Pahima E., Church J., Adam S., Borin V.A., Chazan A., Augustin S., Wietek J., Diene J., Peleg Y., Kawanabe A., Fujiwara Y., Yizhar O., Sheves M., Schapiro I., Furutani Y., Hideki K., Keeichi I., Hegemann P., Béjà O., Shalev-Benami
CoChR and CapChR2 2022 Lahore R.G.F, Pampaloni N.P., Peter E., Heim M-M., Tillert L., Vierock J., Oppermann J., Walther J., Schmitz S., Owald D., Plested A.J.R, Rost B.R., Hegemann P.
Human a7 nicotinic receptor 2023 Prevost M.S, Barilone N., Dejean de la Batie G., Pons S., AYME G., Patrick E., Marc G., BonTems F., Pehau-Arnaudet G., Maskos U., Lafaye P., Corringer P-j.
CCR5 2023 Gu S., Huang M., Handel T.M.

Lab Results

Rho1D4-tag purification products are especially handy when it comes to the purification of proteins with low abundance. And a huge group of these proteins is membrane proteins. Even overexpressed their concentration does not come close to their soluble counterparts.

Figure 1 shows the purification of four different membrane proteins. Each time the purification has resulted in great purity and lots of protein.
Examples for RHo1D4-tag protein purifications using Cube Biotech products
Figure 1: Example of Rho1D4-tag purifications using Rho1D4 MagBeads. The four depicted anti-Rho1D4 western blots show one different mutant of the SARS-CoV-2 surface protein SPIKE. A: Alpha mutant B.1.1.7 B: Beta mutant B.1.351 C: Epsilon mutant B.1.429 D: Eta mutant B.1.525

FAQ

Can I get the datasheet for the Highspec Rho1D4 Agarose?

Can I regenerate & reuse your Glutathione beads?

Can I use the Rho1D4-tag also for non-membrane proteins?

Yes, you can.

I never heard about the Rho1D4-tag before. What can you tell me about it?

Do not worry. All your questions should be answered on our Rho1D4-tag knowledge page.

I use the FLAG-tag (DYKDDDDK) for my protein purifications. Is it beneficial for me to switch to the Rho1D4-tag?