Cu-NTA

Cu-NTA is a term that refers to a copper ion that has been coupled to nitrilotriacetic acid (NTA). Cu-NTA can then be coupled to agarose or magnetic beads for Immobilized Metal Chelate Affinity Chromatography, short IMAC. This is a purification method for two purposes:

  1. The purification of his tagged proteins
  2. The enrichment of copper binding proteins