Product information "PureCube Compact Cartridge Cu-NTA"
Type:
Cartridge
Bead size:
40 µm (Agarose)
Affinity :
Copper Binding Proteins, His-tag
Purpose:
Protein Purification
Ligand:
NTA
Coupled Ion:
Cu2+
PureCube Cu-NTA COMPACT columns / cartridges were designed for convenient FPLC purification of proteins that carry a His-tag tag or the enrichment of copper-binding proteins. The columns come pre-filled with our PureCube Cu-NTA agarose beads (40 µm in diameter). The PureCube COMPACT cartridge was developped with the intent of maximum compability with most commonly used FPLC hardware. It is still recommend to check the given features table . In case that no compability is given, we have another option. Please have a look at our other PureCube Cu-NTA cartridges / columns.
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Features
Usage
Specific binding and purification of 6x His-tagged proteins Enrichment of copper binding proteins
Specifity
Affinity to His-tagged proteins Affinity to copper-binding proteins
Bead Ligand
Cu-NTA
Bead size
40 μm
pH stability/span>
2-14
Chelator stability
Stable in buffer containing 10 mM DTT and 1 mM EDTA
Other stabilities
100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
Filling quantity
Delivered as a 50 % suspension
Features - Regarding Cartridge / Column itself.
Feature
PureCube Compact Cartridge 1 mL
PureCube Compact Cartridge 5 mL
Bed Volume
1 mL
5 mL
Dimensions: diameter X length (mm)
8.0 x 35
17 x 35
Body material
Polypropylene
End Plug Material
Polypropylene
Inlet
10-32 UNF female thread
Outlet
10-32 UNF female thread
Max. Flow Rate
6 mL/min
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