StrepTactin

The Strep-tag® purification system is based on the highly selective binding of engineered streptavidin, called Strep-Tactin, to Strep-tag II fusion proteins. Cube Biotech offers a high-quality affinity resin for Strep-tagged proteins, with maximum binding capacity. Choose your preferred method from either agarose resins or magnetic beads.

Overview of Cube Biotech's Strep®-tag affinity products for Strep®-tag protein purification.


HiCap StrepTactin Agarose (100 µm)	HiCap StrepTactin Agarose (100 µm) Cartridge	HiCap StrepTactin Magnetic Beads
HiCap StrepTactin Agarose (~400 µm)	HiCap StrepTactin Agarose (100 µm) COMPACT Cartridge	HiCap StrepTactin Magnetic Beads XL

Features

Usage	Specific binding and purification of Strep-tagged® proteins
Specifity	Affinity to Strep-tag®
Binding capacity	5 mg/mL
Stability	2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-gluyopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sacrosine, 0,1% SDS, 0,3% CHAPS, 1M Guanidine Hcl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH₄)SO₄, 1M CaCl₂, 25% Glycerol
Bead Ligand	StrepTactin®

Strep-tag® products from Cube Biotech were successfully used in the following publications:

Protein	Year	Author	Product
NPTX1	2020	Sticco M.J., Palomino P.A.P., Lukacsovich D., Thompson B.L., Földy C., Ressl S., Martinelli D.C.¹	PureCube StrepTactin HiCap Magnetic Beads
Sarm1	2021	Li Y., Pazyra-Murphy M.F., Avizonis D., de Sá Tavares Russo M., Tang S., Chen C-Y., Hsueh Y-P., Bergholz S.J., Jiang T., Zhao J. J:, Zhu J., Woo Ko K., Milbrandt J., DiAntonio A., Segal R.A. ²	PureCube StrepTactin HiCap Magnetic Beads
NMNAT	2022	Dingwall C.B ., Strickland A., Yum S.W., Yim A.K., Zhu J., Wang P.L., Yamada Y., Schmidt R.E, Sasaki Y., Bloom A.J., DiAntonio A., Milbrandt J. ³	PureCube StrepTactin HiCap Magnetic Beads
TIR domains and TIR domain-encoding proteins	2022	Weagley J.S., Zaydman M., Venkatesh S., Sasaki Y., Damaraju N., Yenkin A., Buchser W., Rodionov D.M. Osterman A., Ahmed T., Barratt M.J., DiAntonio A., Milbrandt J., Gordon J.I. ⁴	PureCube StrepTactin HiCap Magnetic Beads

References

Sticco, MJ, Peña Palomino, PA, Lukacsovich, D, et al. C1QL3 promotes cell‐cell adhesion by mediating complex formation between ADGRB3/BAI3 and neuronal pentraxins. The FASEB Journal. 2021; 35:e21194.
Li Y . et al. Sarm1 activation produces cADPR to increase intra-axonal Ca++ and promote axon degeneration in PIPN. J Cell Biol (2022) 221; doi.org/10.1083/jcb.202106080
Dingwall C.B et al. Macrophage depletion blocks congenital SARM1-dependent neuropathy. BioxRiv-Preprint Server (2022) 221; https://doi.org/10.1101/2022.02.26.482110

StrepTactin® and Strep-tag® are trademarks of IBA GmbH. PureCube StrepTactin Agarose is manufactured by IBA GmbH under German Patent Application No. 42 37 113.9 entitled "Fusion peptides with binding activity for streptavidin".