The Strep-tag® purification system is based on the highly selective binding of engineered streptavidin, called Strep-Tactin, to Strep-tag II fusion proteins. Cube Biotech offers a high-quality affinity resin for Strep-tagged proteins, with maximum binding capacity. Choose your preferred method from either agarose resins or magnetic beads.
Overview of Cube Biotech's Strep®-tag affinity products for Strep®-tag protein purification.
|Usage ||Specific binding and purification of Strep-tagged® proteins |
|Specifity ||Affinity to Strep-tag® |
|Binding capacity ||5 mg/mL |
|Stability ||2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-gluyopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sacrosine, 0,1% SDS, 0,3% CHAPS, 1M Guanidine Hcl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH4)SO4, 1M CaCl2, 25% Glycerol |
|Bead Ligand ||StrepTactin® |
Strep-tag® products from Cube Biotech were successfully used in the following publications:
|NPTX1 ||2020 ||Sticco M.J., Palomino P.A.P., Lukacsovich D., Thompson B.L., Földy C., Ressl S., Martinelli D.C.1 ||PureCube StrepTactin HiCap Magnetic Beads |
|Sarm1 ||2021 ||Li Y., Pazyra-Murphy M.F., Avizonis D., de Sá Tavares Russo M., Tang S., Chen C-Y., Hsueh Y-P., Bergholz S.J., Jiang T., Zhao J. J:, Zhu J., Woo Ko K., Milbrandt J., DiAntonio A., Segal R.A. 2 ||PureCube StrepTactin HiCap Magnetic Beads |
- Sticco, MJ, Peña Palomino, PA, Lukacsovich, D, et al. C1QL3 promotes cell‐cell adhesion by mediating complex formation between ADGRB3/BAI3 and neuronal pentraxins. The FASEB Journal. 2021; 35:e21194.
- Li Y . et al. et al. Sarm1 activation produces cADPR to increase intra-axonal Ca++ and promote axon degeneration in PIPN. J Cell Biol (2022) 221; doi.org/10.1083/jcb.202106080
StrepTactin® and Strep-tag® are trademarks of IBA GmbH. PureCube StrepTactin Agarose is manufactured by IBA GmbH under German Patent Application No. 42 37 113.9 entitled "Fusion peptides with binding activity for streptavidin".