PureCube HiCap StrepTactin MagBeads

Order number: 34201

€109.00*

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Description

Our PureCube StrepTactin® MagBeads is a suspension of 6% cross-linked magnetic agarose beads that is coupled to the StrepTactin protein. They enable protein yields of up to 7 mg protein per ml MagBeads.

They were developed for protein purification assays using the Strep-tag® using magnetic separation. PureCube StrepTactin® products can also be obtained as agarose resin. Furthermore, PureCube StrepTactin® MagBeads XL are also available for very vicious cell media.

For your convenience Cube Biotech also offers its own MagBead separator for use with our magnetic beads.
Feature
Usage Highly specific affinity purification of Strep-(WSHPQFEK)-tagged proteins
Specificity Affinity to Strep-tagged (WSHPQFEK) proteins
Binding capacity >7 mg/ml
Bead Ligand StrepTactin®
Bead size 30 μm
Matrix 6% cross-linked agarose
Filling quantity Delivered as a 5 % suspension
Chemical stability 2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-gluyopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sacrosine, 0,1% SDS, 0,3% CHAPS, 1M Guanidine HCl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH4)SO4, 1M CaCl2, 25% Glycerol
Short-term storage In equilibration buffer (see protocol)
Long-term storage In 20% ethanol at 4°C

FAQ

Can I get the datasheet for the PureCube HiCap StrepTactin® MagBeads?

Is StrepTactin® the same as Streptavidin?

No, they are not. StrepTactin® is a bioengineered protein, originally based on Streptavidin. However, StrepTactin cannot bind to biotin, while Streptavidin cannot bind to the Strep-tag.

Can I regenerate & reuse your StrepTactin® beads?

Disclaimer

StrepTactin® and Strep-tag® are trademarks of IBA GmbH. PureCube StrepTactin Agarose is manufactured by IBA GmbH under German Patent Application No. 42 37 113.9 entitled "Fusion peptides with binding activity for streptavidin".